| Budget Amount *help |
¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
Fiscal Year 2016: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2015: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2014: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Outline of Final Research Achievements |
In this study, nucleotide sequences of Pk intron (nuclear DNA) in Paragonimus westermani were used to construct phylogenetic trees. As a result, it was discovered that the India and Sri Lankan clades have been diverged from the other Asian clades of P. westermani considerably long time ago. In addition, it became clear that two clades from Malaysia and Philippines was included in the East Asia clade. Phylogenetic tree obtained from this intron region became quite different from the tree using mitochondrial DNA (cox1). Furthermore, it was considered that a lot of mutation of the Pk intron position has been created by the recombination events between different alleles, indicating a single event of triploid origin. Both of the India and Sri Lankan clades could be separate species from P. westermani.
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