| Co-Investigator(Kenkyū-buntansha) |
加藤 恵三 岐阜大学, 医学部附属病院, 講師 (40397336)
米本 和弘 岐阜大学, 医学部附属病院, 助教 (80422731)
安彦 善裕 北海道医療大学, 歯学部, 教授 (90260819)
牧田 浩樹 岐阜大学, 大学院医学系研究科, 講師 (50345790)
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| Budget Amount *help |
¥15,990,000 (Direct Cost: ¥12,300,000、Indirect Cost: ¥3,690,000)
Fiscal Year 2018: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2017: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2016: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2015: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2014: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
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| Outline of Final Research Achievements |
To reveal the DNA methylation status of oral mucosa affected by the betel quid chewing habit using the swab samples in south Asia, we carried out the analysis of lesions in in betel quid chewers. Firstly, we evaluated the swab sample accuracy using swab and tissue samples in promoter region of p16 and MGMT so on. DNA methylation status was well correlated between swab and tissue samples. Then, we analyzed DNA methylation status of Taiwan swab samples using pyrosequencing method focused on p16 and MGMT. 10% samples of oral cancer patients indicated positive. Furthermore, we examined the methylation positive of p16 in cancer patients and detected it in high ratio (almost 100%). These results possibly suggested the usefulness of p16 analysis as a biomarker in oral cancer.
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