Why do organisms oscillate? – Investigation of biological function of glycolytic oscillations in yeast cells -

• Project/Area Number: 26330326
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Life / Health / Medical informatics
• Research Institution: Yokohama National University
• Principal Investigator: Amemiya Takashi 横浜国立大学, 大学院環境情報研究院, 教授 (60344149)
• Co-Investigator(Kenkyū-buntansha): 伊藤 公紀 横浜国立大学, 大学院環境情報研究院, 教授 (40114376) ; 山口 智彦 国立研究開発法人産業技術総合研究所, その他部局等, 研究員 (70358232)
• Research Collaborator: YAMAMOTO TETSUYA 東京都立産業技術高等専門学校, ものづくり工学科, 教授 (00259839) ; SASAKI SATOSHI 東京工科大学, 応用生物学部, 教授 (70262110)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 細胞 / 解糖系 / 振動反応 / 同期現象 / 酵母細胞 / がん細胞 / 解糖系振動 / 代謝 / 同期 / 細胞間コミュニケーション / 解糖系振動反応 / 情報伝達 / 生命機能

Outline of Final Research Achievements

This study has aimed to clarify biological function of oscillations and synchronization of organisms. We experimentally observed glycolytic oscillations in yeast cells at a single cell level, though yeasts were considered not to exhibit the oscillations above a critical cell density. Analyses of a mathematical model of glycolytic oscillations revealed that the efficiency of energy acquisition is about 7 to 8 % higher in the oscillatory glycolysis than in the stationary-state glycolysis. We also revealed that yeast cells encapsulated in alginate microparticles at a high cell-density exhibited complete synchronization in the glycolytic oscillations.Glycolytic oscillations in cervical cancer HeLa cells that have metabolic similarities with yeast cells were also observed at a single cell level for the first time.

Research Products

• [Journal Article] がん（HeLa）細胞の解糖系振動反応の１細胞観察 (2017)
  • Author(s): 雨宮隆，柴田賢一，伊藤良洋，渡邉昌俊，伊藤公紀，山口智彦
  • Journal Title: 信学技報 Volume: 116(437) Pages: 5-8
• [Journal Article] Collective and individual glycolytic oscillations in yeast cells encapsulated in alginate microparticles (2015)
  • Author(s): Amemiya, T., Obase, K., Hiramatsu, N., Itoh, K., Shibata, K., Takinoue, M., Yamamoto, T. & Yamaguchi , T.
  • Journal Title: Chaos Volume: 25 Issue: 6 Pages: 0646061-0646067
  • DOI: 10.1063/1.4921692
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] マイクロカプセル内の酵母細胞における解糖系振動現象および同期現象 (2015)
  • Author(s): 雨宮隆，小長谷耕平，平松直樹，柴田賢一，伊藤公紀
  • Journal Title: 信学技報 Volume: 115 Pages: 15-16
• [Journal Article] Glycolytic synchronization in yeast cells via ATP and other metabolites: Mathematical analyses by two-dimensional reaction-diffusion models (2014)
  • Author(s): Serizawa, H., Amemiya, T., Itoh, K.
  • Journal Title: Natural Science Volume: 6 Issue: 10 Pages: 719-732
  • DOI: 10.4236/ns.2014.610072
  • Peer Reviewed / Open Access
• [Journal Article] 癌細胞における解糖系振動反応 (2014)
  • Author(s): 雨宮 隆
  • Journal Title: 信学技報 Volume: 114 Pages: 21-23
• [Presentation] Glycolytic oscillations in cancer cells (2016)
  • Author(s): Takashi Amemiya, Kenichi Shibata, Yoshihiro Itoh
  • Organizer: 26th Annual Meeting of MRS-J (2016)
  • Place of Presentation: 横浜市開港記念会館（Yokohama）
  • Year and Date: 2016-12-19
  • Int'l Joint Research / Invited
• [Presentation] Electrochemical and Nanoscale Instabilities (2016)
  • Author(s): Tomohiko Yamaguchi
  • Organizer: Gordon Research Conference on Oscillations & Dynamic Instabilities in Chemical Systems
  • Place of Presentation: Stowe (VT, USA)
  • Year and Date: 2016-07-17
  • Int'l Joint Research / Invited
• [Presentation] Intra- and inter-cellular analysis of glycolytic oscillations in cervical cancer HeLa cells (2016)
  • Author(s): Kenichi Shibata, Takashi Amemiya, Masatoshi Watanabe, Kiminori Itoh, Tomohiko Yamaguchi
  • Organizer: Internal Workshop, Symposium on Nonlinear Sciences - The history for 30 years and vision for the future -
  • Place of Presentation: AIST waterfront, Tokyo
  • Int'l Joint Research
• [Presentation] Distance dependency of synchronized glycolytic oscillations in yeast cells encapsulated in alginate microparticles (2016)
  • Author(s): Yu Kawakita, Kenichi Shibata, Takashi Amemiya, Kiminori Itoh, Tomohiko Yamaguchi
  • Organizer: Internal Workshop, Symposium on Nonlinear Sciences - The history for 30 years and vision for the future -
  • Place of Presentation: AIST waterfront, Tokyo
  • Int'l Joint Research
• [Presentation] Matter and life, and beyond (2016)
  • Author(s): Tomohiko Yamaguchi
  • Organizer: Internal Workshop, Symposium on Nonlinear Sciences - The history for 30 years and vision for the future -
  • Place of Presentation: AIST waterfront, Tokyo
  • Int'l Joint Research / Invited
• [Presentation] 大域結合されたβ細胞集団の膜電位バースト (2016)
  • Author(s): Du Yiechen，柴田賢一，真原仁，雨宮隆
  • Organizer: 第26回非線形反応と協同現象研究会
  • Place of Presentation: 明治大学（東京）
• [Presentation] Intra and inter-cellular analysis of glycolytic oscilltions in cervical cancer HeLa cells (2016)
  • Author(s): 柴田賢一，雨宮隆，伊藤良洋，渡邉昌俊，伊藤公紀，山口智彦
  • Organizer: 第26回非線形反応と協同現象研究会
  • Place of Presentation: 明治大学（東京）
• [Presentation] 酵母細胞集団間の解糖系同期現象の距離依存性解析 (2016)
  • Author(s): 川北侑羽，柴田賢一，雨宮隆
  • Organizer: 第26回非線形反応と協同現象研究会
  • Place of Presentation: 明治大学（東京）
• [Presentation] HeLa細胞の解糖系振動反応のpH依存性 (2016)
  • Author(s): 麻野拓摩，柴田賢一，雨宮隆
  • Organizer: 第26回非線形反応と協同現象研究会
  • Place of Presentation: 明治大学（東京）
• [Presentation] 自己組織化とバイオミメティクス (2016)
  • Author(s): 山口智彦
  • Organizer: 第67回コロイドおよび界面化学討論会
  • Place of Presentation: 旭川教育大学（北海道）
  • Invited
• [Presentation] ジャボチンスキーのおくりもの (2016)
  • Author(s): 山口智彦
  • Organizer: 第26回非線形反応と協同現象研究会
  • Place of Presentation: 明治大学（東京）
  • Invited
• [Presentation] Life and Matter, and Beyond (2016)
  • Author(s): Tomohiko Yamaguchi
  • Organizer: 先端科学技術シンポジウム－自己組織化と新しいナノ物質科学－
  • Place of Presentation: 秋葉原プラザ（東京）
  • Int'l Joint Research / Invited
• [Presentation] Metabolic network and glycolytic oscillations in yeast and cancer cells (2015)
  • Author(s): Amemiya, T., Obase, K., Hiramatsu, N., Itoh, K., Shibata, K., Takinoue, M., Yamamoto, T. & Yamaguchi, T.
  • Organizer: Pacifichem 2015
  • Place of Presentation: ホノルル，ハワイ
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] Function and application of glycolytic oscillations in cells (2015)
  • Author(s): Amemiya, T.
  • Organizer: 25th Annual Meeting of MRS-Japan 2015
  • Place of Presentation: 横浜市開港記念会館，横浜
  • Year and Date: 2015-12-08
  • Invited
• [Presentation] 代謝振動反応を指標とした休眠細胞検出法の確立に向けた基礎研究 (2015)
  • Author(s): 伊藤良洋，柴田賢一，伊藤公紀，雨宮隆
  • Organizer: 第25回非線形反応と協同現象研究会
  • Place of Presentation: 旭川医科大学
  • Year and Date: 2015-10-03
• [Presentation] 細胞の階層性ダイナミクスを用いた毒性評価概念の創出 (2015)
  • Author(s): 小長谷耕平，平松直樹，柴田賢一，伊藤公紀，雨宮隆
  • Organizer: 第25回非線形反応と協同現象研究会
  • Place of Presentation: 旭川医科大学
  • Year and Date: 2015-10-03
• [Presentation] 細胞における解糖系振動反応の医療情報への応用 (2015)
  • Author(s): 雨宮隆
  • Organizer: 電子情報通信学会，2015ソサイエティ大会
  • Place of Presentation: 東北大学川内北キャンパス
  • Year and Date: 2015-09-09
  • Invited
• [Presentation] マイクロカプセル内の酵母細胞における解糖系振動現象および同期現象 (2015)
  • Author(s): 雨宮隆，小長谷耕平，平松直樹，柴田賢一，伊藤公紀
  • Organizer: 電子情報通信学会 有機エレクトロニクス研究会
  • Place of Presentation: 大濱信泉記念館，石垣市
  • Year and Date: 2015-04-29
• [Presentation] Glycolytic oscillations in yeast and cancer cells (2014)
  • Author(s): Amemiya, T.
  • Organizer: 24th Annual Meeting of MRS-Japan 2014
  • Place of Presentation: 横浜市開港記念会館（横浜）
  • Year and Date: 2014-12-10 – 2014-12-12
  • Invited
• [Presentation] 酵母解糖系における振動反応の時空間制御 (2014)
  • Author(s): 平松直樹，柴田賢一，伊藤公紀，山本哲也，山口智彦，雨宮隆
  • Organizer: 第24回非線形反応と協同現象研究会
  • Place of Presentation: 東京電気大学（千住）
  • Year and Date: 2014-12-06
• [Presentation] 酵母マイクロカプセル化における解糖系振動反応の酵母間相互作用の解析 (2014)
  • Author(s): 小長谷耕平，柴田賢一，伊藤公紀，瀧ノ上正浩，雨宮隆
  • Organizer: 第24回非線形反応と協同現象研究会
  • Place of Presentation: 東京電気大学（千住）
  • Year and Date: 2014-12-06
• [Presentation] 酵母におけるG0細胞周期と解糖系振動反応の関連性の解析 (2014)
  • Author(s): 伊藤良洋，柴田賢一，伊藤公紀，雨宮隆
  • Organizer: 第24回非線形反応と協同現象研究会
  • Place of Presentation: 東京電気大学（千住）
  • Year and Date: 2014-12-06
• [Presentation] 酵母解糖系振動反応を利用したバイオアッセイの検討 (2014)
  • Author(s): 飯塚一歩，柴田賢一，伊藤公紀，雨宮隆
  • Organizer: 第24回非線形反応と協同現象研究会
  • Place of Presentation: 東京電気大学（千住）
  • Year and Date: 2014-12-06
• [Presentation] 癌細胞における解糖系振動反応 (2014)
  • Author(s): 雨宮 隆
  • Organizer: 電気情報通信学会 有機エレクトロニクス
  • Place of Presentation: 奄美大島商工会議所（鹿児島）
  • Year and Date: 2014-11-28 – 2014-11-29
• [Presentation] Cell-to-cell communication in yeasts via ATP (2014)
  • Author(s): Amemiya, T.
  • Organizer: IUMRS-ICA2014
  • Place of Presentation: 福岡大学（福岡）
  • Year and Date: 2014-08-24 – 2014-08-30
  • Invited
• [Presentation] Metabolic network and glycolytic oscillations in yest and other cells (2014)
  • Author(s): Amemiya, T., Miyata, T., Hiramatsu, N., Shibata, K., Itoh, K.
  • Organizer: Gordon Research Conference, Oscillations & Dynamic Instabilities in Chemical Systems
  • Place of Presentation: Girona, Spain
  • Year and Date: 2014-07-13 – 2014-07-18
• [Funded Workshop] Internal Workshop, Symposium on Nonlinear Sciences - The history for 30 years and vision for the future - (2016)
  • Place of Presentation: AIST Waterfront (Tokyo)
  • Year and Date: 2016-09-07