Iron metabolism and DNA repair,genetic diseases: the roles of CIAX copmplex

• Project/Area Number: 26340022
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: Nigata University of Phermacy and Applied Life Sciences
• Principal Investigator: Seki Mineaki 新潟薬科大学, 健康・自立総合研究機構, 准教授 (40304167)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: DNA修復 / 鉄硫黄クラスター / 修復 / 変異 / 鉄代謝 / 損傷

Outline of Final Research Achievements

To know the roles of iron-sulfur (FeS) cluster in DNA repair related genetic disease, we focused on the putative FeS cluster binding motif in UVSSA protein. We generated multiple mutants and found some of the mutants show sensitivity to UV.The expression levels of CIAX components in cancer cell lines were also examined. CIAX complex is up-regulated in blood cancer cell lines. In particular, CIAX complex is highly expressed in macrophage-like cells such as THP-1 and U937. The reduction of CIAX expression level does not affect phagocytosis.

Research Products

• [Remarks] 新潟薬科大学 健康自立総合研究機構
  • URL: http://www.nupals.ac.jp/institution/kenkoujiritsu.html