| Project/Area Number |
26340027
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation and chemicals
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| Research Institution | Takasaki University of Health and Welfare |
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Principal Investigator |
TANAKA Susumu 高崎健康福祉大学, 健康福祉学部, 教授 (70348142)
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| Co-Investigator(Kenkyū-buntansha) |
保坂 公平 高崎健康福祉大学, 大学院健康福祉学研究科, 講師 (70108992)
山際 教之 高崎健康福祉大学, 薬学部, 教授 (70433638)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | カルシニューリン / アルキルベンゼンスルホン酸 / インターロイキン-2 / 非競合阻害 / 阻害物質 |
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| Outline of Final Research Achievements |
We identified that linear alkylbenzene sulfonate (LAS) from acrylonitrile butadiene rubber, recognized as an environmental pollutant, is an inhibitor of calcineurin (CN)-related activity involved in cellular immunity. Studies on inhibition of CN activity using LAS analogues revealed that the inhibitory effect of LAS increased depending on the length of the alkyl chain and the anionic surfactant restored CN inhibition by LAS. Using a double-reciprocal plot, C12-LAS was determined to have noncompetitive inhibitory effects. Subsequently, we assessed the cellular immunity role of LAS by examining the effects of LAS on interleukin-2 (IL-2) produced by Jurkat cells. It was revealed that ConA-induced IL-2 produced by Jurkat cells was suppressed by LAS via the transcription factor NFAT.
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