| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Outline of Final Research Achievements |
Human Poleta is a major translesion synthesis polymerase involved in DNA damage tolerance against UV- or cisplatin-induced DNA lesions. To elucidate its physiological function and regulatory mechanism in cellular DNA damage tolerance, I focused on unique structural features of human Poleta identified from the comparison of its crystal structure against other eukaryotic TLS polymerases. As a result, unique amino acid sequences or amino acid residues of human Poleta were deleted or substituted by other amino acid to investigate their role. Biochemical and cellular analyses using these mutant proteins revealed that some of them were important for the translesion synthesis activity or regulation of human Poleta. It was notable, in this research, that several novel proteins and chemical compounds were identified to interact with or regulate human Poleta. These findings will open new phase of research on DNA damage tolerance associated with human Poleta.
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