Functional analyses of translesion synthesis DNA polymerase Poleta based on its structural features
Project/Area Number |
26340028
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Risk sciences of radiation and chemicals
|
Research Institution | Kobe University (2016-2017) Gakushuin University (2014-2015) |
Principal Investigator |
YOKOI MASAYUKI 神戸大学, バイオシグナル総合研究センター, 准教授 (00322701)
|
Project Period (FY) |
2014-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
|
Keywords | 損傷乗り越え合成 / DNA損傷 / TLSポリメラーゼ / 阻害剤 / 損傷乗り越え複製 / Polη / 紫外線 |
Outline of Final Research Achievements |
Human Poleta is a major translesion synthesis polymerase involved in DNA damage tolerance against UV- or cisplatin-induced DNA lesions. To elucidate its physiological function and regulatory mechanism in cellular DNA damage tolerance, I focused on unique structural features of human Poleta identified from the comparison of its crystal structure against other eukaryotic TLS polymerases. As a result, unique amino acid sequences or amino acid residues of human Poleta were deleted or substituted by other amino acid to investigate their role. Biochemical and cellular analyses using these mutant proteins revealed that some of them were important for the translesion synthesis activity or regulation of human Poleta. It was notable, in this research, that several novel proteins and chemical compounds were identified to interact with or regulate human Poleta. These findings will open new phase of research on DNA damage tolerance associated with human Poleta.
|
Report
(5 results)
Research Products
(16 results)
-
-
-
-
-
-
[Presentation] Functional analysis of human DNA polymerase η harboring mutations in the palm domain2016
Author(s)
Yokoi, M., Mishima, K., Noda, C., Nishimura, J., Yang, W., Hanaoka, F.
Organizer
第39回 日本分子生物学会
Place of Presentation
神奈川県横浜
Year and Date
2016-11-30
Related Report
-
-
-
-
-
[Presentation] Hypersensitivity of Pols η, ι, and κ triple knockout cells to mutagens is a valuable indicator of genotoxicity2015
Author(s)
150.Akagi, J., Yokoi, M., Cho, Y-M., Toyoda, T., Hanaoka, F., Ogawa, K
Organizer
第74回 日本癌学会学術総会
Place of Presentation
名古屋
Year and Date
2015-10-08
Related Report
-
-
[Presentation] Roles of DNA polymerase η and ι in UV-induced mutagenesis of skin cells and tissues2015
Author(s)
149.Sakurai, Y., Yokoi, M., Tsukamoto, T., Wei, M., Wanibuchi, H., Murakumo, Y., Hanaoka, F.
Organizer
第104回 日本病理学会総会
Place of Presentation
横浜
Year and Date
2015-04-30
Related Report
-
-
-