Glycoscience on medaka development toxicity and innate immune function inhibition by silver nanoparticles

• Project/Area Number: 26340030
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: Toyo University
• Principal Investigator: Kashiwada Shosaku 東洋大学, 生命科学部, 教授 (20370265)
• Co-Investigator(Kenkyū-buntansha): 宮西 伸光 東洋大学, 食環境科学部, 教授 (80372720)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 銀ナノ粒子 / メダカ / 糖鎖 / 免疫 / 生態リスク / 銀ナノコロイド / 魚病菌 / 感染

Outline of Final Research Achievements

Ecotoxicity of nanomaterials is one of the international concerns. Silver nanoparticles, which account for about half of the international market as health and hygiene products, are considered to be one of the most ecological risk chemicals, since they have antibacterial activity in particular. In this study, we investigated the influence of silver nanocolloid (SNC), which is considered to be frequently used as silver nanoparticles, on embryo development and on innate immunity to medaka. From analyses of microarray and quantitative PCR, gene expressions related to immunity, biological defense mucosa etc. were disrupted by SNC exposure. Infection experiments with Edwardsiella tarda revealed that the infection of medaka adults increased due to SNC exposure.

Research Products

• [Journal Article] Three-dimensional Visualization of GFP-labeled Edwardsiella tarda in Whole Medaka (2017)
  • Author(s): Chisato Kataoka, Haruka Tomiyama, Shosaku Kashiwada
  • Journal Title: Journal of Fish Diseases Volume: 40 Issue: 4 Pages: 479-484
  • DOI: 10.1111/jfd.12522
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Salinity-dependent Toxicity Assay of Silver Nanocolloids Using Medaka Eggs (2016)
  • Author(s): Chisato Kataoka, Shosaku Kashiwada
  • Journal Title: Journal of Visualized Experiments Volume: 109 Issue: 109
  • DOI: 10.3791/53550
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Salinity increases the toxicity of silver nanocolloids to Japanese medaka embryos (2015)
  • Author(s): Chisato Kataoka, Tadashi Ariyoshi, Hideo Kawaguchi, Seiji Nagasaka, and Shosaku Kashiwada
  • Journal Title: Environmental Science: Nano Volume: 2 Issue: 1 Pages: 94-103
  • DOI: 10.1039/c4en00175c
  • Peer Reviewed / Open Access
• [Journal Article] Free silver ion as the main cause of acute and chronic toxicity of silver nanoparticles to cladocerans (2015)
  • Author(s): Sakamoto, M., J-Y, Ha, S. Yonashima, C. Kataoka, H. Tatsuta, and S. Kashiwada
  • Journal Title: Archives of Environmental Contamination and Toxicology Volume: 68 Issue: 3 Pages: 500-509
  • DOI: 10.1007/s00244-014-0091-x
  • Peer Reviewed / Open Access
• [Journal Article] Effects of silver nanocolloids on early life stages of the scleractinian coral Acropora japonica (2014)
  • Author(s): Ryota Suwa, Chisato Kataoka, Shosaku Kashiwada
  • Journal Title: Marine Environmental Research Volume: 99 Pages: 198-203
  • DOI: 10.1016/j.marenvres.2014.06.010
  • Peer Reviewed / Open Access
• [Presentation] Toxico-Glycobiology: Silver Nanocolloids effects on Medaka Embryogenesis through Glycosylation Disruption (2016)
  • Author(s): Kaori Shimizu, Misato Fujita, Kensuke Fukao, Futaba Mogi, Yoshiriro Kagami, Nobumitsu Miyanishi, Shosaku Kashiwada
  • Organizer: Society of Environmental Toxicology and Chemistry Asia/Pacific 2016 Conference
  • Place of Presentation: National University of Singapore, Singapore
  • Year and Date: 2016-09-16
  • Int'l Joint Research
• [Presentation] Silver Nanocolloids Disrupt Medaka Immune System and Resistance against a Common Pathogen Edwardsiella tarda (2016)
  • Author(s): Chisato Kataoka, Shotaro Izumi, Misato Fujita, Shosaku Kashiwada
  • Organizer: 30th New European Society for Comparative Physiology and Biochemistry
  • Place of Presentation: Cosmocaixa, Barcelona, Spain
  • Year and Date: 2016-09-04
  • Int'l Joint Research
• [Presentation] Medaka Fish Model for Environmental Health Sciences (2016)
  • Author(s): Shosaku Kashiwada
  • Organizer: International Meeting on Aquatic Model Organisms for Human Disease and Toxicology Research
  • Place of Presentation: Okazaki Conference Center, Okazaki, Japan
  • Year and Date: 2016-03-18
  • Int'l Joint Research / Invited
• [Presentation] Environmental Immuno-Toxicology of Silver Nanocolloids using Medaka (2016)
  • Author(s): Chisato Kataoka, Shotaro Izumi, Haruka Tomiyama, Misato Fujita, Shosaku Kashiwada
  • Organizer: International Meeting on Aquatic Model Organisms for Human Disease and Toxicology Research
  • Place of Presentation: Okazaki Conference Center, Okazaki, Japan
  • Year and Date: 2016-03-18
  • Int'l Joint Research
• [Presentation] Glycosylation Relative Genes as Toxicity Targets of Silver Nanocolloids in Medaka Embryos (2016)
  • Author(s): Kaori Shimizu, Misato Fujita, Kensuke Fukao, Futaba Mogi, Yoshiriro Kagami, Nobumitsu Miyanishi, Shosaku Kashiwada
  • Organizer: International Meeting on Aquatic Model Organisms for Human Disease and Toxicology Research
  • Place of Presentation: Okazaki Conference Center, Okazaki, Japan
  • Year and Date: 2016-03-18
  • Int'l Joint Research
• [Presentation] Silver Nanocolloids Increases Pathogen-Infection Risk (2015)
  • Author(s): Chisato Kataoka, Shotaro Izumi, Misato Fujita, Shosaku Kashiwada
  • Organizer: PRIMO18
  • Place of Presentation: Trondheim, Norway
  • Year and Date: 2015-05-24
  • Int'l Joint Research
• [Presentation] 化学物質生態リスク評価の展望 (2015)
  • Author(s): 柏田祥策
  • Organizer: 第59回日本応用動物昆虫学会研究小集会
  • Place of Presentation: 山形大学小白河キャンパス（山形県山形市）
  • Year and Date: 2015-03-27
  • Invited
• [Presentation] 銀ナノコロイドの水環境生態リスク (2015)
  • Author(s): 柏田祥策
  • Organizer: 株式会社パーキンエルマージャパン主催ナノ粒子分析セミナー「Nanolytica」
  • Place of Presentation: 横浜ビジネスパークウエストタウン（神奈川県横浜市）
  • Year and Date: 2015-02-03
  • Invited
• [Presentation] Aquatic Toxicology on Glycobiology using Medaka―Silver Nanotoxicology (2014)
  • Author(s): Shosaku Kashiwada
  • Organizer: The Medaka Model for Comparative Assessment of Human Disease Mechanisms
  • Place of Presentation: Hilton Inn at Austin-Bergstrom International Airport
  • Year and Date: 2014-12-18
  • Invited
• [Presentation] Medaka Model Study for Immuno-Toxicology using Silver Nanocolloids (2014)
  • Author(s): Chisato Kataoka, Shotaro Izumi, Misato Fujita, Shosaku Kashiwada
  • Organizer: The 7th Aquatic Animal Models of Human Disease Conference
  • Place of Presentation: Hyatt Regency Lost Pines Resort and Spa (Austin, TX, USA）
  • Year and Date: 2014-12-13 – 2014-12-18
• [Presentation] Immune responses of medaka embryos exposed to silver nanocolloids are stage dependent (2014)
  • Author(s): Chisato Kataoka, Shotaro Izumi, Misato Fujita, Shosaku Kashiwada
  • Organizer: 20th Japanese Medaka and Zebrafish Meeting
  • Place of Presentation: Keio University Faculty of Pharmacy, Tokyo, Japan
  • Year and Date: 2014-09-20 – 2014-09-21
• [Presentation] 銀ナノコロイドのメダカ免疫シグナル影響 (2014)
  • Author(s): 片岡知里，泉庄太郎，藤田深里，柏田祥策
  • Organizer: 第20回日本環境毒性学会研究発表会
  • Place of Presentation: 冨山国際会議場（富山県射水市）
  • Year and Date: 2014-09-10 – 2014-09-11
• [Presentation] メダカ受精卵の糖転移酵素遺伝子に対する銀ナノコロイドの影響 (2014)
  • Author(s): 清水香里，深尾研亮，茂木双葉，藤田深里，柏田祥策
  • Organizer: 第20回日本環境毒性学会研究発表会
  • Place of Presentation: 冨山国際会議場（富山県射水市）
  • Year and Date: 2014-09-10 – 2014-09-11
• [Presentation] Silver Nanocolloids Have Impacts on Medaka Innate Immune Responses (2014)
  • Author(s): Chisato Kataoka, Tadashi Ariyoshi, Takuto Niwa, Misato Fujita, Shosaku Kashiwada
  • Organizer: Society of Environmental Toxicology and Chemistry, Twenty-Fourth Annual Meeting in Europe
  • Place of Presentation: Basel, Switzerland
  • Year and Date: 2014-05-11 – 2014-05-15
• [Remarks] 東洋大学生命環境科学研究センター
  • URL: http://www.aqua-env.org/