| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
In this study, we developed novel monitoring system to estimate the effect of low-dose radiation originated from contaminated soil by Fukushima-Daiichi Nuclear accident in 2011. We used plant culturing cells (callus) harboring a monitoring gene constructed by alterative GUS gene. In this system, we can detect the frequency of homologous recombination in somatic cells as the radiation-induced DNA damage by counting GUS spots, and then evaluate the effects of low-dose radiation.
We evaluated radiation effects on plant genome with dose-dependent manner both in experimental condition using contaminated soil and in field condition. As a result, we succeeded in generation of an experimental system to detect DNA damage from the low-dose radiation not only in experimental room condition but in field condition.
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