Development of evaluation method using plant callus with a monitoring gene capable of detecting biological effects of the environmental radiation
| Project/Area Number |
26340044
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Environmental impact assessment
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
玉置 雅紀 国立研究開発法人国立環境研究所, 福島支部, 主席研究員 (00311324)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 放射線影響 / 植物 / シロイヌナズナ / カルス / モニター遺伝子 / ゲノム不安定化 |
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| Outline of Final Research Achievements |
In this study, we developed novel monitoring system to estimate the effect of low-dose radiation originated from contaminated soil by Fukushima-Daiichi Nuclear accident in 2011. We used plant culturing cells (callus) harboring a monitoring gene constructed by alterative GUS gene. In this system, we can detect the frequency of homologous recombination in somatic cells as the radiation-induced DNA damage by counting GUS spots, and then evaluate the effects of low-dose radiation.
We evaluated radiation effects on plant genome with dose-dependent manner both in experimental condition using contaminated soil and in field condition. As a result, we succeeded in generation of an experimental system to detect DNA damage from the low-dose radiation not only in experimental room condition but in field condition.
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Report
(5 results)
Research Products
(19 results)
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[Book] Science, Society, Technology & Sustainable Development in Tunisia and Japan (Proceedings of the 13th Tunisia-Japan Symposium on Science, Society and Technology (TJASSST 2015), held in Tsukuba, Japan, February 2016)2018
Author(s)
Shinya Takahashi, Kei H. Kojo, Natsumaro Kutsuna, Hiroko Isoda, Seiichiro Hasezawa
Publisher
Springer
Related Report
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