| Project/Area Number |
26340067
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Modeling and technologies for environmental conservation and remediation
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| Research Institution | Muroran Institute of Technology |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | Penicillium / 炭化水素 / 根圏土壌 / 低温域 / 南極 / メタゲノム / アルキルフェーノル / 低温分解 / MnPの精製 / Penicillium sp. CHY-2株 / 寒冷地 / DGGE / gene / Gene |
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| Outline of Final Research Achievements |
Biodegradability of eight different aliphatic and aromatic hydrocarbons (octane, decane, dodecane, ethylbenzene, butylbenzene, naphthalene, acenaphthene, and benzo[a]pyrene) was confirmed using Penicillium sp. CHY - 2 isolated from Antarctic soil. In addition, it was found that the enzyme manganese peroxidase (MnP) derived from strain CHY -2 was involved in the degradation of decane which showed the highest degradation rate among the tested aliphatic and aromatic hydrocarbons. Furthermore, various rhizosphere microorganisms were isolated and found to be involved in hydrocarbon decomposition. As a result of confirming the gene of strain CHY-2 responsible for degradation, genes encoding cytochrome P450 hydroxylase, lignin peroxidase and vanillyl alcohol oxidase were confirmed.
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