| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Clostridium paraputrificum isolated from seaside soil is an anaerobic bacterium that produces a large amount of hydrogen gas. Genes encoding lactate dehydrogenase, pyruvate-ferredoxin oxidoreductase, thiolase, phospho-trans-acetylase and phospho-trans-butylase were inactivated by ClosTron method. Each transformant was cultivated anaerobically in 100mL of GS-medium containing glucose as a sole carbon source and hydrogen gas produced during cultivation was analyzed by gas chromatography. The production of the hydrogen gas of the transformant that lacked lactate dehydrogenase became 1.4 times of the wild type strain. Next, we attempted to produce hydrogen gas by C. paraputrificum using cellulose as carbon source. C. paraputrificum cannot use cellulose as a carbon source. Therefore, Ruminiclostridium josui that was isolated from compost was used as cellulose degrader. The mix-culture of both strain was able to produce hydrogen gas from crystalline cellulose.
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