The Use of Laccase Enzyme for Eco-friendly Dyeing and Washing

• Project/Area Number: 26350078
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Clothing life/Dwelling life
• Research Institution: Wayo Women's University
• Principal Investigator: NAGASHIMA Naoko 和洋女子大学, 生活科学系, 准教授 (30459599)
• Research Collaborator: TAKAGISHI Toru 大阪府立大学, 名誉教授
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 環境調和型 / 酵素 / ラッカーゼ / 羊毛 / 反応染料 / 脱色 / 漂白 / 洗浄 / 酸化還元酵素 / 退色 / 界面活性剤 / 移染防止 / 環境調和型染色加工 / 洗剤

Outline of Final Research Achievements

The decomposition and decoloration of hetero bifunctional reactive dyes with an oxidoreductase, laccase were studied. The improvement of wet rubbing fastness of wool fabrics dyed with the reactive dyes and optimum conditions to prevent migration of the dyes in the aqueous washing were also investigated. As a result, the blue dye was found to be decolored rapidly under the conditions at pH 4.5 and 45℃. In addition, the staining on the standard adjacent fabrics in the practical washing was inhibited by using laccase/mediator/nonionic detergent.
The substrate specificity of the laccase for the blue dye was very large and the dye was effectively decolored with the enzyme. Thus it is expected that the enzyme used in this experiment can be applied to the wool dyeing and practical washing.

Research Products

• [Journal Article] ラッカーゼによる異種二官能型反応染料の分解・脱色：羊毛の反応染色への応用 (2017)
  • Author(s): 高岸徹、長嶋直子、平田雄一
  • Journal Title: 繊維・高分子機能加工第120委員会年次報告 Volume: 67 Pages: 40-43
  • Acknowledgement Compliant
• [Journal Article] 水溶液中における異種二官能型反応染料のラッカーゼによる分解・脱色挙動 (2015)
  • Author(s): 高岸徹、長嶋直子
  • Journal Title: 繊維・高分子機能加工第120委員会 年次報告 Volume: 66 Pages: 48-51
  • Acknowledgement Compliant
• [Presentation] ラッカーゼによる異種二官能型反応染料の分解・脱色：羊毛の反応染色への応用 (2016)
  • Author(s): 長嶋直子、平田雄一、高岸徹
  • Organizer: （一社）繊維学会染色研究委員会 第53回染色化学討論会
  • Place of Presentation: 山形大学
  • Year and Date: 2016-09-20
• [Presentation] 酵素の繊維染色加工 (2016)
  • Author(s): 長嶋直子
  • Organizer: （一社）日本家政学会第1回家政学夏季セミナー
  • Place of Presentation: 実践女子大学渋谷キャンパス
  • Year and Date: 2016-08-30
  • Invited
• [Presentation] 羊毛の反応染色におけるラッカーゼの利用 (2016)
  • Author(s): 長嶋直子、高岸徹
  • Organizer: （一社）繊維学会年次大会
  • Place of Presentation: タワーホール船堀（東京）
  • Year and Date: 2016-06-10
• [Presentation] 羊毛の反応染色におけるラッカーゼの利用 (2016)
  • Author(s): 長嶋直子、高岸徹
  • Organizer: （一社）繊維学会 年次大会
  • Place of Presentation: タワーホール船堀（東京）
  • Year and Date: 2016-06-08
• [Presentation] 水溶液中における異種二官能型反応染料のラッカーゼによる分解・脱色挙動 (2015)
  • Author(s): 長嶋直子、高岸徹
  • Organizer: （一社）繊維学会 秋季研究発表会
  • Place of Presentation: 京都工芸繊維大学（京都）
  • Year and Date: 2015-10-22