Study on control method using Campylobacter specific bacteriophages in chicken meat

• Project/Area Number: 26350114
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Eating habits
• Research Institution: Nakamura Gakuen University Junior College
• Principal Investigator: FURUTA Munenori 中村学園大学短期大学部, 食物栄養学科, 講師 (00343731)
• Co-Investigator(Renkei-kenkyūsha): MIYAMOTO TAKAHISA 九州大学, 大学院農学研究院, 教授 (70190816)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: カンピロバクター / 食中毒菌制御 / バクテリオファージ

Outline of Final Research Achievements

This study was conducted to isolate Campylobacter specific lytic phages from retail chicken meats and to clarify method for controlling Campylobacter in chicken meats by using these isolates. A total of 26 phages were effectively isolated from 13 of 15 retail chicken meats by the enrichment method. Of these, phage PHC10 showed active against 31 of the 46 C. jejuni isolates tested. In vitro effects of phage isolates on viability of C. jejuni were investigated. The result showed that these phages reduced viable counts of C. jejuni by 1～3 log after 6～12 h of incubation at 42 °C as compared to the initial counts. Chicken skin inoculated with phage PHC22 was able to reduce viable counts of C. jejuni by 2 log after 1 h of incubation at 4°C by using vacuum packaging as compared to the control.

Research Products

• [Journal Article] 鶏肉および鶏内蔵から分離されたCampylobacter jejuniにおけるRandom Amplified Polymorphic DNA (RAPD) 法および自動化リボタイピング法による遺伝子型別 (2016)
  • Author(s): 古田宗宜，奈須敬之，Hoang Minh Duc，梅木晃一，本城賢一，宮本敬久
  • Journal Title: 日本防菌防黴学会誌 Volume: 44 Pages: 515-519
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] カンピロバクター特異的バクテリオファージの分離と利用に関する研究 (2016)
  • Author(s): 奈須敬之，古田宗宜，Hoang Minh Duc，本城賢一，宮本敬久
  • Organizer: 日本防菌防黴学会 第43回年次大会
  • Place of Presentation: 東京
  • Year and Date: 2016-09-27
• [Presentation] カンピロバクター特異的バクテリオファージの分離と利用に関する研究 (2015)
  • Author(s): 奈須敬之, 古田宗宜, Hoang Minh Duc, 本城 賢一, 宮本 敬久
  • Organizer: 平成27年度（公社）日本栄養・食糧学会九州・沖縄支部 および（公社）日本食品科学工学会西日本支部合同大会
  • Place of Presentation: 沖縄県市町村自治会館
  • Year and Date: 2015-10-31
• [Presentation] Characterization of Campylobacter Bacteriophages Isolated from Chicken Samples (2015)
  • Author(s): Munenori Furuta, Takayuki Nasu, Hoang Minh Duc, Kenichi Honjoh, Takahisa Miyamoto
  • Organizer: International Association for Food Protection: 2015 IAFP Annual Meeting
  • Place of Presentation: Oregon Convention Center（Oregon）
  • Year and Date: 2015-07-28
• [Presentation] カンピロバクター特異的ファージの分離とその性質 (2014)
  • Author(s): 古田宗宜、梅木晃一、Hoang Minh Duc、小田隆弘、本城賢一、宮本敬久
  • Organizer: 日本防菌防黴学会 第41回年次大会
  • Place of Presentation: きゅりあん（東京）
  • Year and Date: 2014-09-25