Development of DNA identification tool in Brassica species applicable to science education in cooperation with school

• Project/Area Number: 26350229
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Science education
• Research Institution: University of Fukui
• Principal Investigator: Nishizawa Toru 福井大学, 学術研究院教育・人文社会系部門(教員養成), 准教授 (80414382)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: アブラナ類 / 分子マーカー / DNAマーカー / 生物教材 / 生物教育 / 理科教育 / 理科教材 / アブラナ / 学校教育 / 環境調査 / DNA多型 / 葉緑体DNA多型 / 遺伝的多型

Outline of Final Research Achievements

To capture molecular markers that are applicable to species identification of three Brassica species (B. rapa, B. napus, B. juncea), I screened variations in nucleotide sequences of chloroplast and nuclear DNA. Detection of DNA polymorphisms were analyzed by PCR-RFLPs (Restriction fragment length polymorphisms) and PCR-SSCP (Single strand conformation polymorphisms), but hypervariable loci that enables us to discriminate three Brassica species could not be captured.