Microbial experiment using insoluble fibroin membrane-immobilized luminescent bacteria

• Project/Area Number: 26350259
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Science education
• Research Institution: Oita National College of Technology
• Principal Investigator: Ninomiya Junko 大分工業高等専門学校, 一般科理系, 准教授 (60632726)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 環境教育 / 発光細菌 / 実験教材 / 毒性試験 / フィブロイン / 固定化担持膜

Outline of Final Research Achievements

We tried to prepare an experimental teaching material to prepare an insoluble fibroin film (hereinafter referred to as IFF) and perform toxicity test using a tube in which luminescent bacteria are immobilized on IFF. V. fischeri (ATCC 49387) showing the requirement for sulfur source showed increased emission relative to the linear alkylbenzene sulfonate (LAS) which is a surfactant. When luminescent bacteria were immobilized on IFF, it was revealed that they showed a sharper reaction than the susceptibility test in solution. It was suggested that the toxicity test with luminescent bacteria immobilized on IFF is effective as a new experimental teaching material for environmental education.

Research Products

• [Journal Article] Control of Aliivibrio fischeri Luminescence and Decrease in Bioluminescence by fungicides (2018)
  • Author(s): Hitomi Kuwahara, Junko Ninomiya, Hiroshi Morita
  • Journal Title: Biocontrol Science Vol 23., No.2, 2018 Volume: Vol 23., No.2,
  • NAID: 130007485540
  • Peer Reviewed
• [Presentation] 発光細菌を用いた界面活性剤LASの毒性評価 (2017)
  • Author(s): ○二宮純子、上野崇寿、森田洋
  • Organizer: 日本化学会第97回春季年会
  • Place of Presentation: 慶応義塾大学
  • Year and Date: 2017-03-16
• [Presentation] Toxicity assay using luminescent bacteria for fungicides (OPPNa, OPP) (2017)
  • Author(s): Junko Ninomiya , Hitomi Kuwahara , Takashi Furukawa , Takahisa Ueno, Hiroshi Morita
  • Organizer: 2017 Global Conference on Engineering and Applied Science (GCEAS)
  • Int'l Joint Research
• [Presentation] Application of luminescence of marine luminous bacteria Aliivibrio fischeri to toxicity assays for postharvest pesticides imazalil and thiabendazole (2017)
  • Author(s): Hitomi Kuwahara, Junko Ninomiya, Chinatsu Nagahama, Hiroshi Morita
  • Organizer: The international research conference on engineering and technology
  • Int'l Joint Research
• [Presentation] Sensitivity of luminescent bacteria in heavy metals(Hg2+,Cu2+,Cd2+) (2017)
  • Author(s): Junko Ninomiya , Takashi Furukawa, Takahisa Ueno, Hiroshi Morita
  • Organizer: The International Research Conference on Engineering and Technology
  • Int'l Joint Research
• [Presentation] RELATIONSHIP BETWEEN CELL GROWTH AND LUMINESCENCE OF USING FOUR MARINE LUMINOUS BACTERIA IN ARTIFICIAL SEA WATER AND SEA WATER MEDIUM (2017)
  • Author(s): Hitomi Kuwahara, Junko Ninomiya, Hiroshi Morita
  • Organizer: International Congress on Chemical, Biological and Environmental Sciences
  • Int'l Joint Research
• [Presentation] 市販の合成洗剤に対する発光細菌の感受性について (2017)
  • Author(s): 吉野佑弥,杉原勇也,上野崇寿,二宮純子,森田洋
  • Organizer: 日本理科教育学会
• [Presentation] フィブロイン溶液の長期保存 (2017)
  • Author(s): 佐藤司怜,二宮純子,森田洋
  • Organizer: 日本理科教育学会
• [Presentation] Relationship between cell growth and luminescence bacteria in artificial sea water and sea water medium (2016)
  • Author(s): ○H. KUWAHARA, J. NINOMIYA, and H. MORITA
  • Organizer: The Water and Environment Technology Conference 2016
  • Place of Presentation: 中央大学
  • Year and Date: 2016-08-27
  • Int'l Joint Research
• [Presentation] 発光細菌を用いた界面活性剤の毒性評価 (2016)
  • Author(s): ○二宮純子、杉原勇也、上野崇寿、森田洋
  • Organizer: 日本理科教育学会第66回全国大会
  • Place of Presentation: 長野大学
  • Year and Date: 2016-08-06
• [Presentation] 海洋性発光細菌を利用した防ばい剤のバイオアッセイ技術 (2016)
  • Author(s): ○長浜千夏、桑原 眸、二宮純子、森田洋
  • Organizer: 日本農芸化学会2016年度大会
  • Place of Presentation: 札幌コンベンションセンター、札幌市産業振興セ ンター
  • Year and Date: 2016-03-27
• [Presentation] フィブロイン-アルギン酸膜へのVibrio fischeriの固定化とその保存方法 (2016)
  • Author(s): ○二宮 純子・古川 隼士・森田 洋
  • Organizer: 日本化学会第96回春季年会京都
  • Place of Presentation: 同志社大学
  • Year and Date: 2016-03-24
• [Presentation] 発光細菌V.fischeriを用いた水質汚染の評価方法 (2016)
  • Author(s): ○平野 瑠唯・杉原 勇也・上野 崇寿・二宮 純子・森田 洋
  • Organizer: 日本化学会第96回春季年会京都
  • Place of Presentation: 同志社大学
  • Year and Date: 2016-03-24
• [Presentation] 発光細菌を用いたバイオアッセイキットの保存性の検討 (2016)
  • Author(s): ○村田加奈子、古川隼士、二宮純子
  • Organizer: 日本水環境学会九州沖縄支部研究発表会
  • Place of Presentation: 佐賀大学
  • Year and Date: 2016-02-27
• [Presentation] RELATIONSHIP BETWEEN CELL GROWTH AND LUMINESCENCE OF MARINE LUMINOUS BACTERIA IN ARTIFICIAL SEA WATER AND SEA WATER MEDIUM (2016)
  • Author(s): ○H. KUWAHARA, J. NINOMIYA, and H. MORITA
  • Organizer: 22nd International Academic Conference on Engineering, Technology and Innovations
  • Place of Presentation: ヒルトン成田
  • Year and Date: 2016-01-15
  • Int'l Joint Research
• [Presentation] TOXICITY TESTING USING MARINE LUMINESCENT BACTERIUM Allivibrio fischeri (2016)
  • Author(s): ○C. NAGAHAMA, H. KUWAHARA, J. NINOMIYA, and H. MORITA
  • Organizer: 22nd International Academic Conference on Engineering, Technology and Innovations
  • Place of Presentation: ヒルトン成田
  • Year and Date: 2016-01-15
  • Int'l Joint Research
• [Presentation] 合成洗剤に対する発光細菌の感受性試験 (2016)
  • Author(s): ○吉野祐弥、杉原勇也、二宮純子、森田洋
  • Organizer: 日本理科教育学会九州支部大会
  • Place of Presentation: 宮崎市民プラザ
• [Presentation] 海洋性発光細菌Allivibrio fischeriを用いた毒性検査 (2015)
  • Author(s): ○長浜千夏、桑原眸、二 宮純子、森田洋
  • Organizer: 微生物学の新たな発展，ゲノムから機能・実用に関する九州シンポジウム
  • Place of Presentation: ANA ホリデイ・イン リゾート 宮崎
  • Year and Date: 2015-12-06
• [Presentation] 海洋性発光細菌Allivibrio fischeriniによる毒性検査 (2015)
  • Author(s): ○長浜千夏、桑原 眸、二宮純子、森田 洋
  • Organizer: 第22回日本生物工学会九州支部宮崎大会
  • Place of Presentation: 宮崎大学
  • Year and Date: 2015-12-05
• [Presentation] Study on application of Allivibrio fischeri for the toxicity test (2015)
  • Author(s): ○C. NAGAHAMA, H. KUWAHARA, J. NINOMIYA, and H. MORITA
  • Organizer: 17th International Conference on Energy, Environmental and Chemical Engineering
  • Place of Presentation: 京都ブライトンホテル
  • Year and Date: 2015-11-12
  • Int'l Joint Research
• [Presentation] Allivibrio fischeri を利用した防ばい剤のバイオアッセイ技術 (2015)
  • Author(s): ○長浜千夏、 桑原眸、二宮純子、森田洋
  • Organizer: 日本防菌防黴学会 第42回年次大会
  • Place of Presentation: 千里ライフサイエンスセンター
  • Year and Date: 2015-09-01
• [Presentation] バイオアッセイによる環境評価法 (2015)
  • Author(s): ○長浜千夏、桑原眸、二宮純子、森田洋
  • Organizer: 福岡県環境教育学会 第18回年会
  • Place of Presentation: 福岡教育大学
  • Year and Date: 2015-08-08
• [Presentation] 絹フィブロイン膜を用いたタンパク質の検出反応 (2015)
  • Author(s): ○二宮純子，森田洋
  • Organizer: 日本理科教育学会九州支部沖縄大会
  • Place of Presentation: 沖縄県市町村自治会館
  • Year and Date: 2015-05-23
• [Presentation] 海洋性発光細菌Allivibrio fischeriを利用した毒性検査 (2015)
  • Author(s): ○長浜千夏、桑原眸、 二宮純子、森田洋
  • Organizer: 日本菌学会第59回大会
  • Place of Presentation: 那覇市ぶんかテンブス館
  • Year and Date: 2015-05-15
• [Presentation] Application to the toxic inspection to the food additive of Allivibrio fischeri (2015)
  • Author(s): ○C. NAGAHAMA, H. KUWAHARA, J. NINOMIYA, and H. MORITA
  • Organizer: 12th Asian Congress of Nutrition Pacifico Yokohama, kanagawa, Japan
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2015-05-14
  • Int'l Joint Research
• [Presentation] 発光細菌を供試生物とした毒性評価に関する基礎的検討 (2015)
  • Author(s): 野仲壮真、二宮純子、古川隼士
  • Organizer: 土木学会
  • Place of Presentation: 琉球大学（沖縄県中頭郡）
  • Year and Date: 2015-03-07
• [Presentation] Luminescence inducer of Allivibrio fischeri under artificial sea water (2015)
  • Author(s): 桑原眸、二宮純子、森田洋
  • Organizer: The 29 th INTERNATIONAL SYMPOSIUM ON OKHOTSK SEA & SEA ICE
  • Place of Presentation: 紋別市民会館、紋別文化センター（北海道紋別市）
  • Year and Date: 2015-02-15 – 2015-02-19
• [Presentation] 海洋性発光細菌の発光因子 (2014)
  • Author(s): 桑原眸、二宮純子、森田洋
  • Organizer: 平成26年室内環境学会学術
  • Place of Presentation: 工学院大学（東京都新宿区）
  • Year and Date: 2014-12-05 – 2014-12-06
• [Presentation] 発光バクテリアの生物発光阻害を用いた毒性試験について (2014)
  • Author(s): 二宮純子、古川隼士、森田洋
  • Organizer: 第14回環境技術学会年次大会
  • Place of Presentation: 京都大学（京都府京都市）
  • Year and Date: 2014-09-05
• [Presentation] 発光細菌を用いた水環境の毒性評価の教材化～「自然環境の保全と科学技術の利用」の実験～ (2014)
  • Author(s): 二宮純子、古川隼士、野仲壮真、森田洋
  • Organizer: 日本理科教育学会全国大会
  • Place of Presentation: 愛媛大学（愛媛県松山市）
  • Year and Date: 2014-08-23
• [Presentation] 発光細菌を供試生物とした毒性試験について (2014)
  • Author(s): 二宮純子、古川隼士、森田洋
  • Organizer: 日本理科教育学会九州支部大会
  • Place of Presentation: 熊本大学（熊本県熊本市）
  • Year and Date: 2014-05-18
• [Presentation] 発光細菌を用いた新規バイオセンサー (2014)
  • Author(s): 恵良真理子、二宮純子、田部井陽介、森田洋
  • Organizer: 第73回農業食料工学会年次大会
  • Place of Presentation: 琉球大学（沖縄県中頭郡）
  • Year and Date: 2014-05-16 – 2014-05-19