Microbial experiment using insoluble fibroin membrane-immobilized luminescent bacteria
| Project/Area Number |
26350259
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Science education
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| Research Institution | Oita National College of Technology |
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Principal Investigator |
Ninomiya Junko 大分工業高等専門学校, 一般科理系, 准教授 (60632726)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 環境教育 / 発光細菌 / 実験教材 / 毒性試験 / フィブロイン / 固定化担持膜 |
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| Outline of Final Research Achievements |
We tried to prepare an experimental teaching material to prepare an insoluble fibroin film (hereinafter referred to as IFF) and perform toxicity test using a tube in which luminescent bacteria are immobilized on IFF. V. fischeri (ATCC 49387) showing the requirement for sulfur source showed increased emission relative to the linear alkylbenzene sulfonate (LAS) which is a surfactant. When luminescent bacteria were immobilized on IFF, it was revealed that they showed a sharper reaction than the susceptibility test in solution. It was suggested that the toxicity test with luminescent bacteria immobilized on IFF is effective as a new experimental teaching material for environmental education.
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Report
(5 results)
Research Products
(32 results)
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[Presentation] 海洋性発光細菌の発光因子2014
Author(s)
桑原眸、二宮純子、森田洋
Organizer
平成26年室内環境学会学術
Place of Presentation
工学院大学（東京都新宿区）
Year and Date
2014-12-05 – 2014-12-06
Related Report
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