Restoration of extinct population using seed tissues of museum plant specimens: Technology development and dissemination of tissue culture method
| Project/Area Number |
26350387
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Cultural assets study and museology
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| Research Institution | Niigata University |
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Principal Investigator |
Shiga Takashi 新潟大学, 人文社会・教育科学系, 准教授 (60435881)
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| Co-Investigator(Kenkyū-buntansha) |
長谷川 匡弘 大阪市立自然史博物館, 学芸課, 学芸員 (80610542)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 植物標本 / 組織培養 / 生物保全 / 絶滅危惧種 |
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| Outline of Final Research Achievements |
I investigated the enzyme activity of the embryo and germination test of herbarium specimens' seeds of 722 specimens of 113 species. As the result, the seeds of 336 specimens (46.54%) of 84 species (64.12%) were alive.
To address tissue culture of specimen's seeds, cultivation tests were conducted under various media and pretreatment conditions, using healthy seeds of Saussurea pulchella and Eleocharis acutangula collected from the wild populations. It was possible to control fungi 100% by using surface disinfection with mercuric chloride and a medium supplemented with 2% of preservative PPM.
Using this method, although seed germination was not confirmed as a result of inoculating herbarium specimen's seeds of extinct populations of these species in the medium, the occurrence of fungi was not confirmed even after 2 weeks.
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Report
(4 results)
Research Products
(16 results)
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[Presentation] 標本の種子は長生きか？2014
Author(s)
平澤優輝・港翼・長谷川匡弘・志賀隆
Organizer
日本植物学会第78回大会
Place of Presentation
明治大学
Year and Date
2014-09-12 – 2014-09-14
Related Report
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