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Development of analytical systems for mouse embryos applied with mechanical stimuli

Research Project

Project/Area Number 26350501
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Biomedical engineering/Biomaterial science and engineering
Research InstitutionOkayama University of Science (2016)
Okayama University (2014-2015)

Principal Investigator

Matsuura Koji  岡山理科大学, 工学部, 准教授 (70443223)

Co-Investigator(Renkei-kenkyūsha) Asano Yuka  岡山理科大学, 工学部・生命医療工学科, 研究補助員 (40726741)
Naruse Keiji  岡山大学, 大学院・医歯薬学総合研究科, 教授 (40252233)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsマウス受精卵 / メカニカルストレス / マイクロ流路 / メカノバイオロジー / 三次元培養 / 人工卵管・子宮
Outline of Final Research Achievements

Molecular mechanisms for responses to mechanical stimuli (MS) in mammalian embryos are dependent on developmental stage of the embryos and/or the organization of macromolecules inside the embryo. In this study, we constructed an analytical system to evaluate the mechanism based on microscopic observations. By holding mouse embryos with a holding pipette inserted into the microchannel, we analyzed changes of cell shape and intracellular calcium concentration based on fluorescence ratio imaging. To record the images of the embryos during culture, we prepared an embryo culture system equipped with a CO2 gas blender and a stage heater under an inverted microscope. Embryos in the two-cell stage developed to blastocysts using this system. Moreover, we developed a microfluidic system, a cell culture method using hydrogel and a method to engineer a cellular layer that can be applied to embryonic culture.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (6 results)

All 2017 2016 2015 2014

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 2 results) Presentation (2 results) Book (2 results)

  • [Journal Article] Relationship between Porcine Sperm Motility and Sperm Enzymatic Activity using Paper-based Devices2017

    • Author(s)
      Koji Matsuura, Han-Wei Huang, Ming-Cheng Chen, Yu Chen, and Chao-Min Cheng
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 46213-46213

    • DOI

      10.1038/srep46213

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Development of Microfluidic Systems for Fabricating Cellular Multilayers2016

    • Author(s)
      K. Matsuura, I. Sugimoto, Y. Kuroda, K. Kadowaki, M. Matsusaki, M. Akashi
    • Journal Title

      Analytical Sciences

      Volume: 32 Issue: 11 Pages: 1171-1176

    • DOI

      10.2116/analsci.32.1171

    • NAID

      130005278004

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 流路内に保持したマウス受精卵内細胞の顕微観察2016

    • Author(s)
      松浦宏治、浅野友香、成瀬恵治
    • Organizer
      第55回日本生体医工学会大会
    • Place of Presentation
      富山
    • Year and Date
      2016-04-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] マウス桑実胚および胚盤胞のせん断応力に対する細胞変化観察2014

    • Author(s)
      浅野友香、松浦宏治、成瀬恵治
    • Organizer
      第66回日本生理学会 中国四国地方会
    • Place of Presentation
      香川
    • Year and Date
      2014-11-01 – 2014-11-02
    • Related Report
      2014 Research-status Report
  • [Book] Point-of-Care Diagnostics - New Progresses and Perspectives2017

    • Author(s)
      Koji Matsuura, Saori Nishina, Yuka Asano
    • Total Pages
      20
    • Publisher
      IAPC-OBP
    • Related Report
      2016 Annual Research Report
  • [Book] メカノバイオロジー、第21章:生殖工学のバイオメカニクス:生理学・メカニクス・治療2015

    • Author(s)
      成瀬恵治、松浦宏治、原鐡晃
    • Total Pages
      368
    • Publisher
      化学同人
    • Related Report
      2015 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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