Evaluation of relationship between dynamic cytoskeletal rearrangement and intracelluylar mechanical field by using in situ observation of stretched cell
| Project/Area Number |
26350503
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | The University of Tokushima |
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Principal Investigator |
Sato Katsuya 徳島大学, 大学院理工学研究部, 講師 (10403651)
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| Co-Investigator(Kenkyū-buntansha) |
南 和幸 山口大学, 創成科学研究科, 教授 (00229759)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | アクチンリモデリング / バイオメカニクス / ストレッチ刺激 / 骨芽細胞 / MEMS / 細胞骨格リモデリング / メカノバイオロジー / 細胞バイオメカニクス / メカノトランスダクション / アクチン細胞骨格 |
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| Outline of Final Research Achievements |
In this study, we suceeded in conducting in situ time-lapse observation of stretched cell with high spatial and temporal resolution by using originally developped cell stretching MEMS device. Deformation dynamics of actin cytoskeleton under stretch application was viualized by the GFP-tagged lifeact polypeptide.
As the result of the experiment, we found that distribution of axial strain in the actin stress fiber was not uniform, and was not in accord with extracellular substrate deformation. We also found possibillity that depolymerization of actin fibers during actin cytoskeletal rearrangement under cyclic stretch application was localized in the peripheral region of the cell.
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Report
(4 results)
Research Products
(14 results)
