| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
We previously reported that apatite-fiber scaffold (AFS) is applicable for soft-tissue engineering. In this study, we cultured an embryonal carcinoma cell line, P19.CL6, in AFS to demonstrate the utility of this approach for providing a substitute for cardiac tissue. Furthermore, we introduced a GCaMP5G expression vector into the cells, for the real-time evaluation of the functional maturation of cardiac tissue. GCaMP5G includes green fluorescent protein (GFP) and calcium-binding domains, and is used as GFP-based calcium indicator. Changes in the fluorescence intensity, which synchronized with the spontaneous beating of the differentiated P19.CL6 cells, were observed. We also examined the cell differentiation after cell culturing in AFS. Markers for cardiac cell differentiation were detected by RT-PCR and real-time PCR; however, no change in fluorescence intensity was observed. It may be due to the complicated cell differentiation in three-dimensional AFS.
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