In vitro fabrication of Human heart tissue model with perfusable blood vessels

• Project/Area Number: 26350517
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Tokyo Women's Medical University
• Principal Investigator: Sekine Hidekazu 東京女子医科大学, 医学部, 講師 (60541737)
• Co-Investigator(Renkei-kenkyūsha): SHIMIZU Tatsuya 東京女子医科大学, 医学部, 教授 (40318100) ; KOBAYASHI Eiji 慶應義塾大学, 医学部 ブリヂストン臓器再生医学寄附講座, 特任教授 (00245044)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 組織モデル / ヒト組織モデル / 心筋細胞 / 細胞シート / 血管網付与

Outline of Final Research Achievements

Creation of 3-D tissue model similar to a biological tissue using tissue engineering method are expected as an alternative approach to 2-D culture or animal test in a research for developing a new medicine or regenerative medicine. We have already developed functional 3-D heart tissue with perfusable blood vessels in vitro using cell sheet based tissue engineering approach. Present study assessed the possibility as 3-D tissue model by performing drug evaluation in the engineered heart tissues. It is now possible to analyze the pharmacological effects such as metabolism and the blood pressure as well as action potential by using vascularized heart tissue model.We believe that our 3-D heart tissue model offers a new possibilities for evaluation of drug efficacy.

Research Products

• [Presentation] Analysis of metabolic activity using the engineered heart tissue model (2017)
  • Author(s): Hidekazu Sekine, Tatsuya Shimizu
  • Organizer: Keystonesymposia Mitochondria Communication
  • Place of Presentation: Taos, New Mexico, USA
  • Year and Date: 2017-01-14
  • Int'l Joint Research
• [Presentation] Ex vivo fabrication of heart tissue model with perfusable blood vessels using cell sheet (2016)
  • Author(s): Hidekazu Sekine, Tatsuya Shimizu
  • Organizer: Basic Cardiovascular Sciences (BCVS) Scientific Sessions 2016
  • Place of Presentation: Phoenix, Arizona, USA
  • Year and Date: 2016-07-18
  • Int'l Joint Research
• [Presentation] Ex vivo engineering of vascularized heart tissue model (2016)
  • Author(s): Hidekazu Sekine, Tatsuya Shimizu
  • Organizer: European Chapter Meeting of the Tissue Engineering and Regenerative Medicine International Society 2016
  • Place of Presentation: Uppsala, Sweden
  • Year and Date: 2016-06-28
  • Int'l Joint Research
• [Presentation] Fabrication of vascularized 3-D heart tissue model for drug evaluation (2015)
  • Author(s): Hidekazu Sekine, Tatsuya Shimizu
  • Organizer: World Conference on Regenerative Medicine
  • Place of Presentation: Congress Center Leipzig
  • Year and Date: 2015-10-21
  • Int'l Joint Research
• [Presentation] The Model of Vascularized Three Dimensional Heart Tissue for Drug Evaluation (2015)
  • Author(s): Hidekazu Sekine, Tatsuya Shimizu
  • Organizer: 2015 4th TERMIS World Congress
  • Place of Presentation: The Boston Marriott Copley Place
  • Year and Date: 2015-09-08
  • Int'l Joint Research