Development of high contrast fluorescence microscopy using enhanced fluorescence of an optical interference mirror slide

• Project/Area Number: 26350518
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Tokyo University of Technology
• Principal Investigator: Akimoto Takuo 東京工科大学, 応用生物学部, 教授 (90367194)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: バイオセンサー / 蛍光増強 / 蛍光顕微鏡

Outline of Final Research Achievements

An enhanced fluorescence can be observed with an optical interference mirror slide consisting of a metal and a dielectric layer. However, a general fluorescence microscopy does not provide the enhanced fluorescence because of the polarization dependence of the enhanced fluorescence. To obtain the enhanced fluorescence, we developed a fluorescence microscope equipped with a concentric circular polarized excitation. As a result, 42-fold enhanced fluorescence was achieved by the microscope with the concentric circular polarized excitation.

Research Products

• [Journal Article] 蛍光増強効果をもつ薄膜干渉基板のバイオセンサーへの応用 (2015)
  • Author(s): 秋元卓央、安田充
  • Journal Title: 色材協会誌 Volume: 88 Pages: 181-186
  • NAID: 130005098852
  • Peer Reviewed
• [Journal Article] High-Contrast Fluorescence Imaging Based on the Polarization Dependence of the Fluorescence Enhancement Using an Optical Interference Mirror Slide (2015)
  • Author(s): Mitsuru Yasuda and Takuo Akimoto
  • Journal Title: Analytical Sciences Volume: 31 Issue: 3 Pages: 139-143
  • DOI: 10.2116/analsci.31.139
  • NAID: 130004827464
  • ISSN: 0910-6340, 1348-2246
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] High-Contrast Fluorescence Microscopy for a Biomolecular Analysis Based on Polarization Techniques Using an Optical Interference Mirror Slide (2014)
  • Author(s): Mitsuru Yasuda and Takuo Akimoto
  • Journal Title: Biosensors Volume: 4 Issue: 4 Pages: 513-522
  • DOI: 10.3390/bios4040513
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 薄膜干渉基板を用いた腫瘍マーカータンパク質のラマン分光分析 (2016)
  • Author(s): 安田充、秋元卓央、尾崎幸洋
  • Organizer: 第76回分析化学討論会
  • Place of Presentation: 岐阜大学（岐阜県、岐阜市）
  • Year and Date: 2016-05-28
• [Presentation] Development of a high-contrast fluorescence microscopy based on polarization techniques using an optical interference mirror slide (2015)
  • Author(s): Yasuda Misturu, Takuo Akimoto
  • Organizer: International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Sheraton Waikiki Hotel （ハワイ,アメリカ合衆国）
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] 薄膜干渉基板を用いた蛍光増強DNAマイクロアレイ (2015)
  • Author(s): 安田充、秋元卓央
  • Organizer: 第76回応用物理学会秋季学術講演会
  • Place of Presentation: 名古屋国際会議場（愛知県名古屋市熱田区）
  • Year and Date: 2015-09-13
• [Presentation] 薄膜干渉基板を用いた偏光技術に基づく高コントラスト蛍光顕微鏡の開発とタンパク質分析への応用 (2015)
  • Author(s): 安田充，秋元卓央
  • Organizer: 第62回 応用物理学会春季学術講演会
  • Place of Presentation: 東海大学（神奈川県、平塚市）
  • Year and Date: 2015-03-11 – 2015-03-14
• [Book] ヘルスケアを支えるバイオ計測 (2016)
  • Author(s): 植田充美監修、秋元卓央他52名
  • Total Pages: 258
  • Publisher: シーエムシー