Formation of functional 3D tissues suitable for transplantation by liver and pancreatic cells
| Project/Area Number |
26350530
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Biomedical engineering/Biomaterial science and engineering
|
|---|
| Research Institution | Chiba University (2016-2018)
Tokyo Women's Medical University (2014-2015) |
|---|
Principal Investigator |
Utoh Rie 千葉大学, 大学院工学研究院, 日本学術振興会特別研究員(RPD) (70593169)
|
|---|
| Research Collaborator |
YAMADA Masumi 千葉大学, 大学院工学研究院, 准教授
SEKI Minoru 千葉大学, 大学院工学研究院, 教授
YAMATO Masayuki 東京女子医科大学, 先端生命医科学研究所, 教授
|
|---|
| Project Period (FY) |
2015-03-01 – 2019-03-31
|
| Project Status |
Completed (Fiscal Year 2018)
|
| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Keywords | 肝細胞 / 膵島細胞 / 組織工学 / コラーゲン / 細胞外マトリックス / 移植 / 膵島組織 / 肝組織 / 膵島 / ハイドロゲル / 細胞治療 |
|---|
| Outline of Final Research Achievements |
We have developed new strategies for production of high functional three-dimensional (3D) tissues using hepatocytes and islet cells that can be efficiently engrafted and function in vivo. First, size-controlled islet-like tissues were prepared from dispersed rat islet cells using precisely-fabricated agarose gel-based microwells. As a result of the culture of islet-like tissues for 7 days, dead cells were rarely present in the small islet-like tissues and the islet cells restored the high insulin secretory capacity in response to high glucose. These results clearly suggest that precise size control of islet-like cells is essential for maintaining islet cell function and survival. In addition, we produced microscale scaffolds with spherical or linear structure from type I collagen and Matrigel for reconstitution of high-functional 3D tissues and evaluated the effects of the microscale scaffolds on survival and functions of hepatocytes.
|
| Academic Significance and Societal Importance of the Research Achievements |
肝疾患や1型糖尿病治療のために，肝細胞移植，膵島移植などの細胞移植療法が試みられているが，現状では，顕著な治療効果が得られない，または長期成績が不良であるなど克服すべき課題が多い。本研究では，細胞移植による治療効果の改善を目指し，肝細胞や膵島細胞を用いて，生体内での長期生着・機能維持に有利な，機能性3次元組織体の作製を行った。移植効率が改善できることによって，移植する細胞数を減らすこともでき，ドナー不足の改善にも期待できる。
|
Report
(5 results)
Research Products
(75 results)
-
-
[Journal Article] Adult hepatocytes direct liver organogenesis through non-parenchymal cell recruitment in the kidney.2018
Author(s)
Utoh R, Komori J, Kuge H, Tatsumi K, Yamada M, Hirohashi S, Tsutsumi M, Amanuma T, Yoshioka A, Nakajima Y, Wake K, Okano T, Lagasse E, Ohashi K.
-
Journal Title
J Hepatol.
Volume: 68
Issue: 4
Pages: 744-753
DOI
Related Report
Peer Reviewed / Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
