| Project/Area Number |
26350530
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biomaterial science and engineering
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| Research Institution | Chiba University (2016-2018)
Tokyo Women's Medical University (2014-2015) |
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Principal Investigator |
Utoh Rie 千葉大学, 大学院工学研究院, 日本学術振興会特別研究員(RPD) (70593169)
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| Research Collaborator |
YAMADA Masumi 千葉大学, 大学院工学研究院, 准教授
SEKI Minoru 千葉大学, 大学院工学研究院, 教授
YAMATO Masayuki 東京女子医科大学, 先端生命医科学研究所, 教授
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| Project Period (FY) |
2015-03-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 肝細胞 / 膵島細胞 / 組織工学 / コラーゲン / 細胞外マトリックス / 移植 / 膵島組織 / 肝組織 / 膵島 / ハイドロゲル / 細胞治療 |
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| Outline of Final Research Achievements |
We have developed new strategies for production of high functional three-dimensional (3D) tissues using hepatocytes and islet cells that can be efficiently engrafted and function in vivo. First, size-controlled islet-like tissues were prepared from dispersed rat islet cells using precisely-fabricated agarose gel-based microwells. As a result of the culture of islet-like tissues for 7 days, dead cells were rarely present in the small islet-like tissues and the islet cells restored the high insulin secretory capacity in response to high glucose. These results clearly suggest that precise size control of islet-like cells is essential for maintaining islet cell function and survival. In addition, we produced microscale scaffolds with spherical or linear structure from type I collagen and Matrigel for reconstitution of high-functional 3D tissues and evaluated the effects of the microscale scaffolds on survival and functions of hepatocytes.
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| Academic Significance and Societal Importance of the Research Achievements |
肝疾患や1型糖尿病治療のために，肝細胞移植，膵島移植などの細胞移植療法が試みられているが，現状では，顕著な治療効果が得られない，または長期成績が不良であるなど克服すべき課題が多い。本研究では，細胞移植による治療効果の改善を目指し，肝細胞や膵島細胞を用いて，生体内での長期生着・機能維持に有利な，機能性3次元組織体の作製を行った。移植効率が改善できることによって，移植する細胞数を減らすこともでき，ドナー不足の改善にも期待できる。
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