| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
To achieve the silence specific genes, a novel type of polyplexes was developed in the absence of polycations in this study. The polyplexes were prepared from poly(lactic acid) with a terminal amino group by the solvent diffusion method. The polyplexes exhibited stable nucleic acids retention in the serum, compared with conventional polyplexes formed from polycations, and sustained release of nucleic acids along with degrade of the polymer. The polyplexes also exhibited low cytotoxicity, long retention of nucleic acids in the cultured cells and gene silencing activity. These results suggested that the polyplexes could help to advance the clinical application for gene therapy.
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