Identification of novel biomarkers for insulin resistance
| Project/Area Number |
26350922
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied health science
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| Research Institution | Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology |
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Principal Investigator |
Fujita Yasunori 地方独立行政法人東京都健康長寿医療センター(東京都健康長寿医療センター研究所), 東京都健康長寿医療センター研究所, 主任研究員 (30515888)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | インスリン抵抗性 / バイオマーカー |
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| Outline of Final Research Achievements |
To identify novel biomarkers for insulin resistance, we performed a comprehensive gene expression analysis of C2C12 myotubes exposed to the saturated fatty acid, palmitate. We identified 38 genes encoding extracellular proteins whose expression was induced in response to palmitate. Among those genes, we focused on Ccl7 and Gdf15 as candidate genes of the biomarker. We measured serum concentrations of CCL7 and GDF15 in Zucker rats, a widely used animal model of obesity and type 2 diabetes. The serum levels of CCL7 and GDF15 were increased in Zucker-fatty rats compared with Zucker-lean rats. Moreover, serum CCL7 levels were positively correlated with body weight. On the other hand, serum GDF15 levels were strongly correlated with parameters of lipid and carbohydrate metabolism and renal function. Further studies are needed to determine the potential usefulness of serum CCL7 and GDF15 as novel biomarkers for insulin resistance.
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Report
(4 results)
Research Products
(6 results)
