| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
To identify novel biomarkers for insulin resistance, we performed a comprehensive gene expression analysis of C2C12 myotubes exposed to the saturated fatty acid, palmitate. We identified 38 genes encoding extracellular proteins whose expression was induced in response to palmitate. Among those genes, we focused on Ccl7 and Gdf15 as candidate genes of the biomarker. We measured serum concentrations of CCL7 and GDF15 in Zucker rats, a widely used animal model of obesity and type 2 diabetes. The serum levels of CCL7 and GDF15 were increased in Zucker-fatty rats compared with Zucker-lean rats. Moreover, serum CCL7 levels were positively correlated with body weight. On the other hand, serum GDF15 levels were strongly correlated with parameters of lipid and carbohydrate metabolism and renal function. Further studies are needed to determine the potential usefulness of serum CCL7 and GDF15 as novel biomarkers for insulin resistance.
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