Role of intestinal flora and its related metabolites in influences of high-fat diet on intestinal diseases

• Project/Area Number: 26350960
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biomolecular chemistry
• Research Institution: Kobe University
• Principal Investigator: NISHIUMI SHIN 神戸大学, 医学研究科, 特命准教授 (20514706)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 高脂肪食 / メタボロミクス / 代謝物 / 腸管腫瘍 / 腸炎 / 腸内細菌 / 大腸がん / 腸疾患

Outline of Final Research Achievements

The aim of this study was to investigate whether the successive intake of high-fat diet affects the pathogenesis of intestinal tumor and inflammatory bowel disease, and to find the candidates of intestinal flora-related metabolites that contribute to the pathogenesis of intestinal tumor and inflammatory bowel disease. As a result, I could suggest the possibility that quality of the fat affects the pathogenesis more greatly than quantity of the fat. In addition, I could clarify the possibility that sarcosine, which is one of amino acids, is one of the candidates of intestinal flora-related metabolites that contribute to the pathogenesis of intestinal tumor.

Research Products

• [Journal Article] The use of on-line SFE-SFC/MS/MS to analyze disease biomarkers in dried serum spots compared with serum analysis using LC/MS/MS. Rapid Communications in Mass Spectrometry (2017)
  • Author(s): Suzuki M., Nishiumi S., Kobayashi T., Sakai A., Iwata Y., Uchikata T., Izumi Y., Azuma T., Bamba T., Yoshida M.
  • Journal Title: Rapid Communications in Mass Spectrometry Volume: 31 Issue: 10 Pages: 886-984
  • DOI: 10.1002/rcm.7857
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 特集「オミックスと栄養学のパラダイムチェンジ」 メタボローム最新の話題-腸疾患に対するメタボローム (2017)
  • Author(s): 西海 信、東 健、吉田 優
  • Journal Title: 医と食 Volume: 9 Pages: 18-21
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Evaluation of a novel system for analyzing hydrophilic blood metabolites (2017)
  • Author(s): Nishiumi S., Shima K., Azuma T., Yoshida M.
  • Journal Title: Journal of Bioscience and Bioengineering Volume: in press Issue: 6 Pages: 754-759
  • DOI: 10.1016/j.jbiosc.2017.01.015
  • NAID: 40021226009
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Investigations in the possibility of early detection of colorectal cancer by gas chromatography/triple-quadrupole mass spectrometry (2017)
  • Author(s): Nishiumi S., Kobayashi T., Kawana S., Unno Y., Sakai T., Okamoto K., Yamada Y., Sudo K., Yamaji T., Saito Y., Kanemitsu Y., Tsuda Okita N., Saito H., Tsugane S., Azuma T., Ojima N., Yoshida M.
  • Journal Title: Oncotarget Volume: 8 Issue: 10 Pages: 17115-17126
  • DOI: 10.18632/oncotarget.15081
  • NAID: 120006373784
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Novel serum metabolomics-based approach by gas chromatography/triple quadrupole mass spectrometry for detection of human skin cancers: candidate biomarkers (2017)
  • Author(s): Fukumoto T., Nishiumi S., Fujiwara S., Yoshida M., Nishigori C.
  • Journal Title: The Journal of Dermatology Volume: in press
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] LC-MS/MS-based metabolome analysis detected changes in the metabolic profiles of small and large intestinal adenomatous polyps in ApcMin/+ mice (2016)
  • Author(s): Suzuki M., Nishiumi S., Kobayashi T., Azuma T., Yoshida M.
  • Journal Title: Metabolomics Volume: 12 Issue: 4 Pages: 1-9
  • DOI: 10.1007/s11306-016-0988-7
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Abstracts of the 25th Annual Conference of APASL, February 20-24, 2016, Tokyo, Japan. (2016)
  • Author(s): Nababan S., Kawano Y., Nishiumi S., Yano Y., Yoshida M., Azuma T.
  • Journal Title: Hepatology International Volume: Suppl 1 Issue: S1 Pages: 1-506
  • DOI: 10.1007/s12072-016-9707-8
  • Peer Reviewed
• [Presentation] 家族性大腸腺腫症モデルApcMin/+マウスにおける腸管ポリープのメタボローム解析 (2016)
  • Author(s): 鈴木誠、西海信、小林隆、東健、吉田優
  • Organizer: 第10回メタボロームシンポジウム
  • Place of Presentation: 鶴岡メタボロームキャンパス（山形・鶴岡）
  • Year and Date: 2016-10-19
• [Presentation] Ether phospholipid profile in mice with non-alcoholic steatohepatitis (2016)
  • Author(s): Saut Nababan, Yuki Kawano, Shin Nishiumi, Yoshihiko Yano, Masaru Yoshida, Takeshi Azuma
  • Organizer: The 25th Asian Pacific Association for the Study of the Liver (APASL 2016)
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2016-02-20
  • Int'l Joint Research
• [Presentation] 「アミノ酸研究の新展開：細胞シグナルとしての動的制御機構」ワークショップ：APC遺伝子変異による大腸癌の代謝プロファイル変動と細胞増殖に対する影響 (2015)
  • Author(s): 吉田優, 西海信, 吉江智郎, 東健
  • Organizer: 第38回日本分子生物学会年会、第88回日本生化学会大会 合同大会（BMB2015）
  • Place of Presentation: 兵庫
  • Year and Date: 2015-12-01
  • Invited
• [Presentation] 高脂肪食摂取が引き起こす腸内環境の変化に対するメタボローム解析による評価 (2015)
  • Author(s): 西海信, 吉田優.
  • Organizer: 第40回 日本医用マススペクトル学会年会
  • Place of Presentation: 静岡
  • Year and Date: 2015-09-16
• [Presentation] メタボロミクスによる非アルコール性脂肪性肝炎に対する治療薬の探索 (2014)
  • Author(s): 西海 信、寺島 禎彦、東 健、吉田 優
  • Organizer: 第39回日本医用マススペクトル学会年会
  • Place of Presentation: 千葉
  • Year and Date: 2014-10-16 – 2014-10-17
• [Presentation] Metabolome Analysis-Based Attempt to Discover Therapeutic Agents for Non-Alcoholic Steatohepatitis (2014)
  • Author(s): Yoshihiko Terashima, Shin Nishiumi, Namiko Hoshi, Masaru Yoshida, Takeshi Azuma
  • Organizer: AGA-DDW2014
  • Place of Presentation: Chicago, USA
  • Year and Date: 2014-05-04 – 2014-05-06
• [Remarks] 神戸大学大学院医学研究科内科学講座消化器内科学分野ホームページ
  • URL: http://www.med.kobe-u.ac.jp/gi/index.html