Elucidation of structure-function relationship of HSPB1 in anticancer drug resistance mechanism

• Project/Area Number: 26350963
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biomolecular chemistry
• Research Institution: Osaka Medical College
• Principal Investigator: SAKAI AKIKO 大阪医科大学, 医学部, 講師 (30225750)
• Co-Investigator(Kenkyū-buntansha): 田中 覚 大阪医科大学, 医学部, 非常勤講師 (50595741)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: HSPB1 / 抗癌剤耐性 / サイトケラチン / オリゴマー構造 / リン酸化

Outline of Final Research Achievements

The heat-shock protein (HSP) B1 expression increased with the acquisition of 5-fluorouracil resistance. We analyzed the phosphorylation and oligomeric structure of HSPB1 in anticancer agent 5-fluorouracil and paclitaxel-resistant cells, demonstrated that the oligomeric structures and phosphorylation of HSPB1 in both resistant cells are defferent. In addition, cytokeratin subtypes and other two proteins were identified as proteins that interact with HSPB1.

Research Products

• [Journal Article] A phase II, multicenter, single-arm trial of eribulin as first- or second-line chemotherapy for HER2-negative advanced or metastatic breast cancer: evaluation of efficacy, safety, and patient-reported outcomes (2018)
  • Author(s): Kimura Kosei、Iwamoto Mitsuhiko、Tanaka Satoru、Yamamoto Daigo、Yoshidome Katsuhide、Ogura Hiroyuki、Terasawa Risa、Matsunami Nobuki、Takahashi Yuko、Nitta Toshikatsu、Morimoto Takashi、Fujioka Hiroya、Kawaguchi Kanako、Uchiyama Kazuhisa
  • Journal Title: Cancer Chemotherapy and Pharmacology Volume: 81 Issue: 5 Pages: 923-933
  • DOI: 10.1007/s00280-018-3567-y
  • Peer Reviewed
• [Journal Article] Comparative proteomic analysis of paclitaxel resistance-related proteins in human breast cancer cell lines. (2017)
  • Author(s): 藤岡大也
  • Journal Title: ONCOLOGY LETTERS Volume: 13 Issue: 1 Pages: 289-295
  • DOI: 10.3892/ol.2016.5455
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Phase II Study of Neoadjuvant Anthracycline-Based Regimens Combined With Nanoparticle Albumin-Bound Paclitaxel and Trastuzumab for Human Epidermal Growth Factor Receptor 2-Positive Operable Breast Cancer. (2015)
  • Author(s): Tanaka S, Iwamoto M, Kimura K, Matsunami N, Morishima H, Yoshidome K, Nomura T, Morimoto T, Yamamoto D, Tsubota Y, Kobayashi T, Uchiyama K.
  • Journal Title: Clin Breast Cancer Volume: 15 Issue: 3 Pages: 191-196
  • DOI: 10.1016/j.clbc.2014.12.003
  • Peer Reviewed / Open Access
• [Journal Article] Down-regulation of collagen I biosynthesis in intestinal epithelial cells exposed to indomethacin: a comparative proteome analysis. (2014)
  • Author(s): Edogawa S, Sakai A et al
  • Journal Title: Journal of Proteomics Volume: 103 Pages: 35-46
  • DOI: 10.1016/j.jprot.2014.03.022
  • Peer Reviewed
• [Journal Article] Breast conserving surgery using volume replacement with oxidized regenerated cellulose: a cosmetic outcome analysis. (2014)
  • Author(s): Tanaka S, Sato N, Fujioka H, Takahashi Y, Kimura K, Iwamoto M, Uchiyama K.
  • Journal Title: Breast J Volume: 20 Pages: 154-158
  • Peer Reviewed
• [Presentation] 抗癌剤耐性株における熱ショック蛋白質HSPB1の翻訳後修飾とオリゴマー構造 (2014)
  • Author(s): 境晶子，林秀行
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2014-11-25 – 2014-11-27