| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Outline of Final Research Achievements |
RNA switches, which change theirs conformation in response to binding of their target ligand and subsequently interact with Tat-peptide, were rationally designed by considering stabilities of RNA conformations before and after the ligand binding. Tat-peptide is derived from trans activator of transcription (Tat) protein, which activates transcription upon binding to its target RNA. Thus, the RNA switches would be functional units for constructing transcription regulation system in response to a specific target molecule.
In addition, possibility of the RNA switch for translation regulation was investigated. Translation efficiency of a reporter mRNA depended on whether its 5′ untranslated region is forming G-quadruplex or maturely exclusive hairpin. Translation regulation would be possible by controlling the equilibrium between the G-quadruplex and hairpin structures that would applicable for multistep gene regulation together with the transcription regulation system as mentioned above.
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