The role of neuronal diversity in the neural circuit of somatosensory cortex

• Project/Area Number: 26350980
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Basic / Social brain science
• Research Institution: Osaka University
• Principal Investigator: HIRAYAMA Teruyoshi 大阪大学, 生命機能研究科, 特任講師(常勤) (40437398)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 神経細胞 / 多様性 / 神経回路形成 / 大脳皮質 / 遺伝子改変マウス / 回路形成 / 体性感覚野 / クラスター型プロトカドヘリン

Outline of Final Research Achievements

Using several c-Pcdh deletion mutant and conditional knockout（cKO） mouse lines, I investigated the role of c-Pcdh in the somatosensory cortical barrel formation. Pcdh-alpha, Pcdh-beta and Pcdh-alpha-beta deletion mutant mice showed normal barrel formation. Pcdh-gamma deletion mutant mice died within 24h after birth. I analyzed Pcdh-gamma cKO mice and found that obscure barrel formation. Based on these results, I generated c-Pcdh gene modified mouse using CRISPR/Cas9 system.

Research Products

• [Journal Article] Genome Stability by DNA polymerase β in Neural Progenitors Contributes Neuronal Differentiation in Cortical Development (2017)
  • Author(s): Kohei Onishi, Akiko Uyeda, Mistuhiro Shida, Teruyoshi Hirayama, Takeshi Yagi, Nobuhiko Yamamoto, Noriyuki Sugo
  • Journal Title: Journal of Neuroscience Volume: 37 Issue: 35 Pages: 8444-8485
  • DOI: 10.1523/jneurosci.0665-17.2017
  • Peer Reviewed
• [Journal Article] The methyltransferase SETDB1 regulates a large neuron- specific topological chromatin domain. (2017)
  • Author(s): Jiang Y, Loh YE, Rajarajan P, Hirayama T, Liao W, Kassim BS, Javidfar B, Hartley BJ, Kleofas L, Park RB, Labonte B, Ho SM, Chandrasekaran S, Do C, Ramirez BR, Peter CJ, C W JT, Safaie BM, Morishita H, Roussos P, Nestler EJ, Schaefer A, Tycko B, Brennand KJ, Yagi T, Shen L, Akbarian S.
  • Journal Title: Nature Genetics Volume: 49 Issue: 8 Pages: 1239-1250
  • DOI: 10.1038/ng.3906
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Distinct and Cooperative Functions for the Protocadherin-alfa, -beta and -ganma Clusters in Neuronal Survival and Axon Targeting. (2016)
  • Author(s): Hasegawa S, Kumagai M, Hagiwara M, Nishimaru H, Hirano K, Kaneko R, Okayama A, Hirayama T, Sanbo M, Hirabayashi M, Watanabe M, Hirabayashi T, Yagi T
  • Journal Title: Front Mol Neurosci, Volume: 9 Pages: 155-155
  • DOI: 10.3389/fnmol.2016.00155
  • Peer Reviewed / Open Access
• [Journal Article] Establishment of high reciprocal connectivity between clonal cortical neurons is regulated by the Dnmt3b DNA methyltransferase and clustered protocadherins. (2016)
  • Author(s): Tarusawa E., Sanbo M., Okayama A., Miyashita T., Kitsukawa T., Hirayama T., Hirabayashi T., Hasegawa S., Kaneko R., Toyoda S., Kobayashi T., Kato-Itoh M., Nakauchi H., Hirabayashi M., Yagi T., Yoshimura Y.
  • Journal Title: BMC Biology Volume: 14 Issue: 1 Pages: 103-103
  • DOI: 10.1186/s12915-016-0326-6
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Developmental epigenetic modification regulates stochastic expression of clustered protocadherin genes, generating single neuron diversity. (2014)
  • Author(s): Toyoda S, Kawaguchi M, Kobayashi T, Tarusawa E, Toyama T, Okano M, Oda M, Nakauchi H, Yoshimura Y, Sanbo M, Hirabayashi M, Hirayama T, Hirabayashi T, Yagi T.
  • Journal Title: Neuron Volume: 82 Issue: 1 Pages: 94-108
  • DOI: 10.1016/j.neuron.2014.02.005
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] プロトカドヘリンγは大脳皮質抑制性神経細胞の分布及び機能を制御する (2016)
  • Author(s): 有賀理瑛、平山晃斉、吉武講平、足澤悦子、吉村由美子、渋木克栄、八木健
  • Organizer: 第38回日本生物学的精神医学会 第59回日本神経化学会大会 合同年会
  • Place of Presentation: 福岡国際会議場（福岡県福岡市）
  • Year and Date: 2016-09-08
• [Presentation] 生後発生過程の大脳皮質においてpolβ欠損細胞は２本鎖DNA切断を異常に蓄積させる (2015)
  • Author(s): 植田尭子、大西公平、菅生紀之、豊田俊輔、平山晃斉、八木健、山本亘彦
  • Organizer: 38回日本分子生物学会年会 第88回日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアイランド（ 兵庫県 神戸市）
  • Year and Date: 2015-12-03
• [Presentation] 大脳皮質神経幹細胞におけるDNA結合因子CTCFの役割 (2015)
  • Author(s): 平山晃斉、Niels Galjart、八木健
  • Organizer: 第38回日本分子生物学会年会 第88回日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアイランド（ 兵庫県 神戸市）
  • Year and Date: 2015-12-02
• [Presentation] CTCF欠失は大脳皮質抑制性神経細胞の分布と神経活動に影響を及ぼす (2015)
  • Author(s): 有賀理恵、谷垣宏美、平山晃斉、吉武講平、足澤悦子、Niels Caljart、吉村由美子、渋木克栄、八木健
  • Organizer: 38回日本分子生物学会年会 第88回日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアイランド（ 兵庫県 神戸市）
  • Year and Date: 2015-12-02
• [Presentation] プルキンエ細胞におけるCTCF欠損は樹状突起にGiant Lamellar Bodyの形成を即す (2015)
  • Author(s): 角岡佑紀 平山晃斉 中山寿子 足澤悦子 崎村建司 Niels Galjart 吉村由美子 橋本浩一 八木健
  • Organizer: 38回日本分子生物学会年会 第88回日本生化学会大会 合同大会
  • Place of Presentation: 神戸ポートアイランド（ 兵庫県 神戸市）
  • Year and Date: 2015-12-02
• [Presentation] CTCFは抑制性神経細胞の発生、発達、移動に重要な因子である (2014)
  • Author(s): 谷垣 宏美、八木 健、木津川 尚史、平山 晃斉、澁木 克栄、吉武 講平、Galjart Niels
  • Organizer: 第３７回日本神経科学学会
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-11 – 2014-09-13