Why malaria infections including non-human primate malaria infection occurred in specific group

• Project/Area Number: 26360029
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Area studies
• Research Institution: Fujita Health University
• Principal Investigator: Maeno Yoshimasa 藤田保健衛生大学, 医学部, 准教授 (70131191)
• Co-Investigator(Kenkyū-buntansha): 中澤 秀介 長崎大学, 熱帯医学研究所, 助教 (20180268) ; 高木 秀和 愛知医科大学, 医学部, 講師 (90288522)
• Research Collaborator: KAWAI Satoru ; MASUDA Gaku ; SHINE Toshihiko ; Quang Nguyen Tuyen ; Culleton Richard ; Marchand Ron P. ; Huffman Michael Alan ; Chinh Vu Duc ; Hung Vu Viet
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: マラリア / 住民 / 媒介蚊 / サルマラリア / 疫学調査 / 分子生物学 / サルマラリア原虫 / ベトナム / マラリア感染 / 国際共同 / 国際共同研究 / マラリア原虫 / 国際研究者交流 / ヒト / 薬剤耐性遺伝子

Outline of Final Research Achievements

Recent molecular epidemiological studies in Vietnam have reported cases of co-infection with Plasmodium falciparum, P. vivax, P. malariae, and P. knowlesi in An. dirus. The commonly found macaques in the forest in the forested areas are suspected to be bitten by the same An. dirus population that bites humans. Our epidemiological study identified six species of malarial parasites in sporozoite-infected An. dirus using PCR, of which P. vivax was the most common, followed by P. knowlesi, P. inui, P. cynomolgi, P. coatneyi, and P. falciparum. Based on a gametocyte analysis, the same allelic gametocyte types were observed in both humans and mosquitoes at similar frequencies. These observations suggest that people who stay overnight in the forests are frequently infection with both human and non-human primate malarial parasites, leading to the emergence of novel zoonotic malaria. Moreover, it is suggested that mosquito vector populations should be controlled and monitored closely.

Research Products

• [Int'l Joint Research] 国立マラリア学・寄生虫学・昆虫学研究所(ベトナム)
• [Int'l Joint Research] NIMPE(ベトナム)
• [Int'l Joint Research] NIMPE(ベトナム)
• [Journal Article] Plasmodium knowlesi and human malaria parasites in Khan Phu, Vietnam: Gametocyte production in humans and frequent co-infection of mosquitoes. (2017)
  • Author(s): Maeno Y, Culleton R, Quang NT, Kawai S, Marchand RP, NakazawaS
  • Journal Title: Parasitology Volume: 144 Issue: 4 Pages: 527-535
  • DOI: 10.1017/s0031182016002110
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Detection of the Plasmodium falciparum Kelch-13 gene P553L mutation in sporozoites isolated from mosquito salivary glands in South-Central Vietnam (2017)
  • Author(s): Maeno Y, Quang NT, Culleton R, Kawai S, Masuda G, Hori K, Nakazawa S, Marchand RP
  • Journal Title: Parasit Vectors Volume: 10(1) Issue: 1 Pages: 308-308
  • DOI: 10.1186/s13071-017-2247-9
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Molecular epidemiology of mosquitoes for the transmission of forest malaria in south-central Vietnam (2017)
  • Author(s): Maeno Y
  • Journal Title: Trop Med Health. Volume: 45 Issue: 1 Pages: 27-27
  • DOI: 10.1186/s41182-017-0065-6
  • Peer Reviewed / Open Access
• [Journal Article] Detection of the Plasmodium falciparum Kelch-13 gene P553L mutation in sporozoites isolated from mosquito salivary glands in Southcentral Vietnam (2017)
  • Author(s): Maeno Y, Quang NT, Culleton R, Kawai S, Hori K, Nakazawa S, Marchand RP.
  • Journal Title: Parasites & Vectors Volume: 印刷中
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Humans frequently exposed to a range of non-human primate malaria parasite species through the bites of Anopheles dirus mosquitoes in South-central Vietnam (2015)
  • Author(s): Maeno Y, Quang NT, Culleton R, Kawai S, Masuda G, Nakazawa S, Marchand RP
  • Journal Title: Parasites and Vectors Volume: 8 Issue: 1 Pages: 376-376
  • DOI: 10.1186/s13071-015-0995-y
  • NAID: 120005947150
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Detection of Plasmodium knowlesi DNA in the urine and faeces of a Japanese macaque (Macaca fuscata) over the course of an experimentally induced infection (2014)
  • Author(s): Kawai S, Sato M, Kato-Hayashi S, Kishi H, Huffman M, Maeno Y, Culleton R, Nakazawa S
  • Journal Title: Malaria Journal Volume: 13 Issue: 1 Pages: 373-373
  • DOI: 10.1186/1475-2875-13-373
  • NAID: 120006986377
  • Peer Reviewed / Open Access
• [Journal Article] DNA from pre-erythrocytic stage malaria parasites is detectable by PCR in the faeces and blood of hosts. (2014)
  • Author(s): Abkallo HM, Liu W, Hokama S, Ferreira PE, Nakazawa S, Maeno Y, Quang NT, Kobayashi N, Kaneko O, Huffman MA, Kawai S, Marchand RP, Carter R, Hahn BH, Culleton R.
  • Journal Title: Int J Parasitol Volume: 44 Pages: 467-473
  • Peer Reviewed / Open Access
• [Presentation] ベトナム中南部におけるアルテミシニン耐性熱帯熱マラリア原虫の検出とその伝播 (2016)
  • Author(s): 前野芳正, Culleton R, Quang NT, 川合覚, Marchand RP, 中澤秀介
  • Organizer: 第85回日本寄生虫学会大会
  • Place of Presentation: 宮崎市、宮崎市民プラザ
  • Year and Date: 2016-03-19
• [Presentation] ベトナム南部におけるスポロゾイトからみた森林マラリア伝播の分子疫学的研究 (2015)
  • Author(s): 前野芳正、Richard Cullenton、Ngueyen Tuyen Quang、川合覚、伊藤誠、Ron P. Marchand、中澤秀介
  • Organizer: 第84回日本寄生虫学会大会
  • Place of Presentation: 杏林大学三鷹キャンパス
  • Year and Date: 2015-03-21 – 2015-03-22
• [Presentation] Performance of a new LAMP Kit in community-wide screening of asymptomatic malaria (2014)
  • Author(s): Kawai S, Sato M, Maeno Y, Nakazawa S, Xangsayarath P, Pongvongsa T
  • Organizer: The 8th National Health Research Forum
  • Place of Presentation: Vientiane, Lao PDR
  • Year and Date: 2014-10-16 – 2014-10-17