Radiosensitization effect of liposome-encapsulated fullerenes on human melanoma
| Project/Area Number |
26390017
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Nanomaterials chemistry
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| Research Institution | Mie University |
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Principal Investigator |
Kato Shinya 三重大学, 地域イノベーション推進機構, 助教 (40545547)
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| Co-Investigator(Kenkyū-buntansha) |
吉村 哲郎 三重大学, 工学研究科, 特任教授(研究担当) (30035472)
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| Research Collaborator |
MIWA Nobuhiko 県立広島大学, 名誉教授
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | リポソーム / フラーレン / 脂質過酸化 / 膜脂質過酸化 / 脂質膜モデル / 低線量放射線 / 放射線増感剤 / がん細胞 / エックス線 / ヒトメラノーマ / 細胞増殖 / γ-シクロデキストリン / 包接体 / スクワレン |
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| Outline of Final Research Achievements |
The polyhydroxy small-gap fullerenes (SGFs: C120O30(OH)30・30H2O・25Na+) were encapsulated in MLV liposomes (Lpsm) composed of DOPC and DOPS, which are designated as LpsmSGFs (DOPC/DOPS/SGFs = 35 mM:15 mM:246-445 μM, φ141.2 nm, ζ-potential-35.65 mV). Radiosensitization by LpsmSGFs under X-ray irradiation was evaluated on human melanoma HMV-II cells. On 7th day after X-ray irradiation, cell proliferation degree decreased on cells pretreated with LpsmSGFs than Lpsm or free-SGFs. LpsmSGFs obviously exhibited more augmented mitochondrial membrane potentials on perinuclear region of cells than Lpsm or free-SGFs. The oxidation-reduction potentials of SGFs aqueous solution increased by X-ray irradiation. These results suggest that LpsmSGFs-mediated generation of reactive oxygen species results in damages to cellular components such as mitochondria and DNA on cells, and thereby cell proliferation decreased. The LpsmSGFs has a potential as a pro-oxidative type radiosensitizer.
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Report
(5 results)
Research Products
(3 results)
