Development of novel techniques for single-molecule fluorescence imaging of various biomolecules
| Project/Area Number |
26390035
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Nanobioscience
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| Research Institution | Waseda University |
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Principal Investigator |
TANII Takashi 早稲田大学, 理工学術院, 教授 (20339708)
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| Co-Investigator(Kenkyū-buntansha) |
山本 英明 東北大学, 学際科学フロンティア研究所, 助教 (10552036)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 1分子イメージング / 蛍光観察 / FRET / ナノ構造 / １分子イメージング / ＦＲＥＴ / ＺＭＷ / ZMW / 一分子イメージング |
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| Outline of Final Research Achievements |
In order to enhance the ability of single-molecule fluorescence imaging (SMFI) that enables to detect binding and dissociation events of bio-molecules in real time using zero-mode waveguides (ZMWs), we propose a novel technique to combine fluorescence resonance energy transfer (FRET) and SMFI with ZMWs, and proved experimentally that the proposed method is feasible. We also tried to extend the ability of this technique by demonstrating SMFI of individual DNA aptamers for sensing application, SMFI of DNA strand scission by endonuclease, and in-situ cell patterning.
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Report
(4 results)
Research Products
(48 results)
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[Journal Article] Quantitative Evaluation of Cancer Cell Adhesion to Self-Assembled Monolayer-Patterned Substrates by Reflection Interference Contrast Microscopy2016
Author(s)
4)T. Matsuzaki, K. Ito, K. Masuda, E. Kakinuma, R. Sakamoto, K. Iketaki, H. Yamamoto, M. Suganuma, N. Kobayashi, S. Nakabayashi, T. Tanii, H. Y. Yoshikawa
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Journal Title
Journal of Physical Chemistry B
Volume: 120
Issue: 7
Pages: 1221-1227
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Quantitative comparison of cancer and normal cell adhesion using organosilane monolayer templates: An experimental study on the anti-adhesion effect of green-tea catechins2016
Author(s)
Rumi Sakamoto, Eisuke Kakinuma, Kentaro Masuda, Yuko Takeuchi, Kosaku Ito, Kentaro Iketaki, Takahisa Matsuzaki, Seiichiro Nakabayashi, Hiroshi Y. Yoshikawa, Hideaki Yamamoto, Yuko Sato, Takashi Tanii
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Journal Title
In Vitro Cellular & Developmental Biology -Animal
Volume: 52
Issue: 8
Pages: 799-805
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Presentation] モジュール構造型培養神経回路の計算論的モデリング2017
Author(s)
山本 英明, 守谷 哲, 井手 克哉, 松村 亮佑, 秋間 学尚, 久保田 繁, 谷井 孝至, 佐藤 茂雄, 庭野 道 夫, 平野 愛弓
Organizer
第64回応用物理学会春季学術講演会
Place of Presentation
パシフィコ横浜，横浜
Year and Date
2017-03-14
Related Report
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