| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Outline of Final Research Achievements |
Insect olfactory receptors compose heteromeric ion channels activated by volatile organic compounds. Thus, these ion channels transduce the chemical signals into the electrical cell membrane activity. The purpose of this study is development of a new integrative biosensor by using ligand-gated ion channels.
Ion channels were reconstituted by using heterologous gene expression systems. Then, ion channel expressing cell spheroids were cultured in PDMS microchamber. To measure their olfactory response, spheroids were transferred into the hydrogel chamber. Activation of ion channels by odorants was successfully recorded by using extracellular field potential recording technique. The study also suggested the effect of extracellular components in oflactory mucus or lymph on odor recognition.
The research results were published from Angewandte Chemie International Edition.
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