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Development of in vitro embryo culture system based-on microfluidics for artificial reproductive technology

Research Project

Project/Area Number 26390039
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Nano/Microsystems
Research InstitutionTokai University

Principal Investigator

Kimura Hiroshi  東海大学, 工学部, 准教授 (40533625)

Co-Investigator(Renkei-kenkyūsha) FUJII TERUO  東京大学, 生産技術研究所, 教授 (30251474)
OGAWA TAKEHIKO  横浜市立大学, 生命医科学研究科, 教授 (50254222)
Research Collaborator NAKAMURA HIROKO  東海大学, 工学部, 特定研究員
TAKAHASHI TSUBASA  東海大学, 工学研究科, 大学院生
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsマイクロデバイス / 不妊治療 / 受精卵培養 / 精子選別 / 媒精 / 生殖補助医療 / 体外受精
Outline of Final Research Achievements

We aimed to develop an in vitro embryo production device which allows three steps of sperm selection, fertilization and culture on a microdevice. We have integrated the sperm sorting function combining with swim-up and swim-down methods onto the device. To evaluate the device functions, sperm sorting experiment, in vitro fertilization experiment, and embryo culture experiment have been carried out. As results, we concluded that the device has ability to produce high quality embryos by integrating the sperm sorting function. This concept will open and enhance the management of an individual embryo for assisted reproductive technology, livestock breeding, and fundamental stage research by further development.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (29 results)

All 2018 2017 2016 2015 2014 Other

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Acknowledgement Compliant: 3 results,  Open Access: 1 results) Presentation (19 results) (of which Int'l Joint Research: 3 results,  Invited: 11 results) Remarks (5 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Organ/Body-on-a-chip based on Microfluidic technology for drug discovery2018

    • Author(s)
      Hiroshi Kimura, Yasuyuki Sakai, Teruo Fujii
    • Journal Title

      Drug Metabolism and Pharmacokinetics

      Volume: 印刷中

    • NAID

      210000187285

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Long-term ex vivo maintenance of testis tissues producing fertile sperm in a microfluidic device2016

    • Author(s)
      M. Komeya, H. Kimura, H. Nakamura, T. Yokonishi, T. Sato, K. Kojima, K. Hayashi, K. Katagiri, H. Yamanaka, H. Sanjo, M. Yao, S. Kamimura, K. Inoue, N. Ogonuki, A. Ogura, T. Fujii, T. Ogawa
    • Journal Title

      Scientific Reports

      Volume: 6 Issue: 1 Pages: 21472-21472

    • DOI

      10.1038/srep21472

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] マイクロ流体デバイス技術によるOrgan/Body-on-a-chipの実現に向けた取り組み2016

    • Author(s)
      木村啓志,酒井康行,藤井輝夫
    • Journal Title

      月刊「細胞」

      Volume: 48 Pages: 148-148

    • NAID

      40020848143

    • Related Report
      2015 Research-status Report
  • [Journal Article] An On-chip Small Intestine-Liver Model for Pharmacokinetic Studies2015

    • Author(s)
      H. Kimura, T. Ikeda, H. Nakayama, Y. Sakai, T. Fujii
    • Journal Title

      Journal of Laboratory Automations

      Volume: 印刷中 Issue: 3 Pages: 265-273

    • DOI

      10.1177/2211068214557812

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 精子選別機能を有する体外受精卵作製マイクロデバイスの開発2017

    • Author(s)
      高橋翼,中村寛子,木村啓志
    • Organizer
      IIP2017 情報・知能・精密機器部門(IIP部門)講演会
    • Place of Presentation
      東洋大学白山キャンパス、文京区
    • Year and Date
      2017-03-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] マイクロ流体デバイス技術を応用したin vitroモデル~医工連携の試み~2016

    • Author(s)
      木村啓志
    • Organizer
      東海大学総合医学研究所第12回研修会
    • Place of Presentation
      東海大学伊勢原キャンパス、伊勢原市
    • Year and Date
      2016-10-29
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Organ/Body-on-a-Chip実現に向けた試み2016

    • Author(s)
      木村啓志
    • Organizer
      CBI学会2016年大会
    • Place of Presentation
      タワーホール船越、江戸川区
    • Year and Date
      2016-10-25
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] マイクロ流体デバイス技術を応用したOrgan-on-a-Chipの試み2016

    • Author(s)
      木村啓志
    • Organizer
      川島カンファレンス
    • Place of Presentation
      内藤記念くすり博物館、各務原市
    • Year and Date
      2016-10-22
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Microfluidic Spatiotemporal Control Technology for Organ-on-a-Chip2016

    • Author(s)
      Hiroshi Kimura
    • Organizer
      Organ-on-a-chip World Congress 2016
    • Place of Presentation
      Boston, MA, USA
    • Year and Date
      2016-07-07
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 精子選別機能を有する受精卵作製・培養マイクロデバイスの開発2016

    • Author(s)
      高橋翼,中村寛子,木村啓志
    • Organizer
      化学とマイクロ・ナノシステム学会
    • Place of Presentation
      東京大学生産技術研究所、目黒区
    • Year and Date
      2016-04-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] 高品質受精卵作製のための媒精用マイクロデバイスの開発2016

    • Author(s)
      高橋翼, 中村寛子, 木村啓志
    • Organizer
      東海大学マイクロ・ナノ啓発会[Tμne]第6回学術講演会
    • Place of Presentation
      東海大学清水校舎
    • Year and Date
      2016-02-19
    • Related Report
      2015 Research-status Report
  • [Presentation] 精子選別機能を有する受精卵作製マイクロデバイスの開発2015

    • Author(s)
      高橋翼,中村寛子,木村啓志
    • Organizer
      第16回計測自動制御学会システムインテグレーション部門講演会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2015-12-16
    • Related Report
      2015 Research-status Report
  • [Presentation] Microfluidic Environmental Control Technologies for Organ-on-a-chip2015

    • Author(s)
      H. Kimura
    • Organizer
      Lab-on-a-Chip Asia
    • Place of Presentation
      Singapore
    • Year and Date
      2015-11-19
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] マイクロデバイスを用いた精子選別および媒精機能の検討2015

    • Author(s)
      高橋翼, 中村寛子, 木村啓志
    • Organizer
      東海大学マイクロ・ナノ啓発会[Tμne]第5回学術講演会
    • Place of Presentation
      東海大学伊勢原校舎
    • Year and Date
      2015-08-25
    • Related Report
      2015 Research-status Report
  • [Presentation] Microfluidic Spatial Control Technologies for Organ/Body-on-a-chip2015

    • Author(s)
      H. Kimura
    • Organizer
      Organ-on-a-chip World Congress
    • Place of Presentation
      Boston, MA, USA
    • Year and Date
      2015-07-08
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 精子選別機能を有する媒精用マイクロデバイス2015

    • Author(s)
      高橋翼,中村寛子,木村啓志
    • Organizer
      日本機械学会関東学生会第54回学生員卒業研究発表講演会
    • Place of Presentation
      横浜国立大学(神奈川県横浜市)
    • Year and Date
      2015-03-20
    • Related Report
      2014 Research-status Report
  • [Presentation] 精子選別機能を有する媒精デバイス2015

    • Author(s)
      高橋翼,中村寛子,木村啓志
    • Organizer
      東海大学マイクロ・ナノ啓発会[Tμne]第4回学術講演会
    • Place of Presentation
      東海大学(神奈川県平塚市)
    • Year and Date
      2015-02-26
    • Related Report
      2014 Research-status Report
  • [Presentation] 精巣組織片培養のためのマイクロ流体デバイスの開発2015

    • Author(s)
      中村寛子, 古目谷暢, 藤井輝夫, 小川毅彦, 木村啓志
    • Organizer
      東海大学マイクロ・ナノ啓発会[Tμne]第4回学術講演会
    • Place of Presentation
      東海大学(神奈川県平塚市)
    • Year and Date
      2015-02-26
    • Related Report
      2014 Research-status Report
  • [Presentation] マイクロデバイスの医療・生命科学分野への応用2014

    • Author(s)
      木村啓志
    • Organizer
      東海大学産学連携フォーラム2014
    • Place of Presentation
      横浜情報文化センター(神奈川県横浜市)
    • Year and Date
      2014-12-04
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] マイクロ流体デバイスを用いたバイオ環境操作2014

    • Author(s)
      木村啓志
    • Organizer
      表面技術協会関東支部第88回講演会
    • Place of Presentation
      東京医科歯科大学(東京都文京区)
    • Year and Date
      2014-10-24
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 微小空間を巧みに制御するマイクロ流体デバイスの挑戦2014

    • Author(s)
      木村啓志
    • Organizer
      第33回日本糖質学会年会
    • Place of Presentation
      名古屋大学(愛知県名古屋市)
    • Year and Date
      2014-08-10
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 微細加工技術で作製するマイクロ流体デバイスの基礎と応用2014

    • Author(s)
      木村啓志
    • Organizer
      東海大学マイクロ・ナノ啓発会[Tμne]第3回学術講演会
    • Place of Presentation
      東海大学(神奈川県平塚市)
    • Year and Date
      2014-07-24
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] マイクロ流体デバイスのバイオ応用2014

    • Author(s)
      木村啓志
    • Organizer
      理研セミナー
    • Place of Presentation
      理化学研究所(埼玉県和光市)
    • Year and Date
      2014-04-09
    • Related Report
      2014 Research-status Report
    • Invited
  • [Remarks] 東海大学 木村啓志研究室

    • URL

      http://www.kimura-lab.info/

    • Related Report
      2016 Annual Research Report
  • [Remarks] 東海大学工学部機械工学科

    • URL

      http://www.mech.u-tokai.ac.jp/

    • Related Report
      2016 Annual Research Report
  • [Remarks] 東海大学マイクロ・ナノ研究開発センター

    • URL

      http://www.mnc.u-tokai.ac.jp/?page_id=2&lang=ja

    • Related Report
      2016 Annual Research Report
  • [Remarks] 東海大学工学部機械工学科 木村啓志研究室

    • URL

      http://www.kimura-lab.info/

    • Related Report
      2015 Research-status Report
  • [Remarks] 木村啓志ゼミナール

    • URL

      http://www.mech.u-tokai.ac.jp/laboratory/011.html

    • Related Report
      2014 Research-status Report
  • [Patent(Industrial Property Rights)] 組織片の機能を発現・維持する方法および組織片培養デバイス2014

    • Inventor(s)
      藤井輝夫,木村啓志,小川毅彦,古目谷暢
    • Industrial Property Rights Holder
      (学)東海大学、(一財)生産技術研究奨励会、(公大)横浜市立大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2014-119858
    • Filing Date
      2014-06-10
    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

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