| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
To apply a light-induced chromophore color change of a photo-receptor protein bacteriorhodopsin (bR) to a novel optical memory device, it is necessary that bRs are immobilized as maintaining its structure and function. A poly(vinyl alcohol) (PVA) hydrogel with physical cross-linkages (hydrogen bonding) by the freezing-and-thawing (FT) method was used as an immobilization medium. To investigate effects of the PVA gelation on structure and function of bR in purple membrane (PM), spectroscopic techniques were employed against PM/PVA immobilized samples. Time-resolved absorption spectroscopy showed that bR photocycle in the immobilized samples was almost identical to that in suspension. Circular dichroism spectroscopy and X-ray diffraction experiments indicated not only retaining of the native-like 2D crystal of bR but PM stacking during gelation. These results showed that the PVA hydrogel is appropriate to immobilize membrane proteins in terms of maintaining their structure and function.
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