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Development of analysis technique for accelerating protein aggregation and crystallization under applied electric fields and the clarification of the mechanism

Research Project

Project/Area Number 26390049
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Applied materials
Research InstitutionFukushima National College of Technology (2016)
Ibaraki National College of Technology (2014-2015)

Principal Investigator

Wakamatsu Takashi  福島工業高等専門学校, 電気工学科, 教授 (80220838)

Co-Investigator(Kenkyū-buntansha) 千葉 薫  茨城工業高等専門学校, 自然科学科, 准教授 (50415775)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywordsタンパク質 / 凝集体 / 結晶化 / 光散乱 / 電場刺激 / フラクタル / 微粒子
Outline of Final Research Achievements

We have developed a new analysis apparatus with the ability to in situ measure forward scattering light at small angle for crystallizing protein solutions under a low applied voltage.
We tracked protein lysozyme aggregation in the pre-crystalline solutions using the developed apparatus. An enhancement of forward scattering intensities was observed for lysozyme solutions with a low-concentration salt under electric fields, which were under not crystallizing condition without applied voltages. We have found that the application of electric fields provides an effect of accelerating a formation of fractal aggregates on the pre-crystalline step.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (16 results)

All 2017 2016 2015 2014 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Acknowledgement Compliant: 3 results,  Open Access: 1 results) Presentation (7 results) Remarks (4 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 1 results)

  • [Journal Article] Low Applied Voltage Effects on Thaumatin Protein Crystallization2016

    • Author(s)
      Takashi Wakamatsu
    • Journal Title

      Transactions of the Materials Research Society of Japan

      Volume: 41 Issue: 1 Pages: 13-15

    • DOI

      10.14723/tmrsj.41.13

    • NAID

      130005126127

    • ISSN
      1382-3469, 2188-1650
    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Method and apparatus for characterization of electric field-induced aggregation in pre-crystalline protein solutions2015

    • Author(s)
      Takashi Wakamatsu
    • Journal Title

      Review of Scientific Instruments

      Volume: 86 Issue: 1

    • DOI

      10.1063/1.4906328

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Forward-Light-Scattering Characterization of Pre-Crystalline Aggregates in Crystallizing Lysozyme Solutions2014

    • Author(s)
      Takashi Wakamatsu
    • Journal Title

      American Journal of Analytical Chemistry

      Volume: 5 Issue: 09 Pages: 581-588

    • DOI

      10.4236/ajac.2014.59065

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 前方光散乱瞬時測定による電場印加におけるタンパク質凝集化の観察2017

    • Author(s)
      若松 孝
    • Organizer
      第64回応用物理学会学術講演会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2017-03-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] Characterization of Electric Field-Induced Aggregation of Lysozyme using In Situ Forward-Light-Scattering Measurement Technique (前方光散乱瞬時計測法によるリゾチームの電場誘起凝集化の評価)2016

    • Author(s)
      若松 孝
    • Organizer
      The 26th Annual Meeting of MRS-Japan 2016 (第26回日本MRS年次大会)
    • Place of Presentation
      横浜情報文化センター(横浜市)
    • Year and Date
      2016-12-19
    • Related Report
      2016 Annual Research Report
  • [Presentation] 前方散乱光瞬時計測によるリゾチーム凝集・結晶化の評価2016

    • Author(s)
      若松 孝
    • Organizer
      第77回応用物理学会学術講演会
    • Place of Presentation
      朱鷺メッセ(新潟市)
    • Year and Date
      2016-09-13
    • Related Report
      2016 Annual Research Report
  • [Presentation] 前方光散乱法による結晶化前段階形成のリゾチーム凝集体のキャラクタリゼーション2015

    • Author(s)
      若松 孝
    • Organizer
      第25回日本MRS年次大会
    • Place of Presentation
      神奈川県民ホール(横浜)
    • Year and Date
      2015-12-08
    • Related Report
      2015 Research-status Report
  • [Presentation] Effects of Applied Electric Field on Protein Crystallization (電場印加によるタンパク質の結晶化効果)2014

    • Author(s)
      Takashi Wakamatsu (若松 孝)
    • Organizer
      The 24th Annual Meeting of MRS-Japan 2014 (第24回日本MRS年次大会)
    • Place of Presentation
      Yokohama Media & Communications Center
    • Year and Date
      2014-12-10 – 2014-12-12
    • Related Report
      2014 Research-status Report
  • [Presentation] 電場印加によるタンパク質凝集・結晶化の促進効果2014

    • Author(s)
      若松孝
    • Organizer
      平成26年度電子情報通信学会信越支部大会
    • Place of Presentation
      信州大学長野キャンパス
    • Year and Date
      2014-10-04
    • Related Report
      2014 Research-status Report
  • [Presentation] 前方光散乱法による塩のリゾチーム結晶化作用の評価2014

    • Author(s)
      若松孝
    • Organizer
      第14回日本蛋白質科学会年会
    • Place of Presentation
      横浜市横浜産貿ホール
    • Year and Date
      2014-06-25 – 2014-06-27
    • Related Report
      2014 Research-status Report
  • [Remarks] 第16回バイオ・ライフサイエンス研究展(BIO tech 2017) アカデミックフォーラム

    • URL

      http://june2017.tems-system.com/exhiSearch/BIOForum/jp/Workshop

    • Related Report
      2016 Annual Research Report
  • [Remarks] JST新技術説明会(高専-材料分野)

    • URL

      https://shingi.jst.go.jp/list/kosen-k/2016_kosen-k.html

    • Related Report
      2016 Annual Research Report
  • [Remarks] イノベーション・ジャパン2016 大学見本市

    • URL

      http://www.jst.go.jp/tt/fair/ij2016/exhibitor/js20160232.html

    • Related Report
      2016 Annual Research Report
  • [Remarks] 茨城高専地域共同テクノセンター 教員研究紹介

    • URL

      http://www.ibaraki-ct.ac.jp/?page_id=3603/

    • Related Report
      2016 Annual Research Report
  • [Patent(Industrial Property Rights)] PCT国際出願2016

    • Inventor(s)
      若松 孝
    • Industrial Property Rights Holder
      国立高等専門学校機構
    • Industrial Property Rights Type
      特許
    • Filing Date
      2016-10-03
    • Related Report
      2016 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] ①結晶化分析装置及び結晶化分析方法2015

    • Inventor(s)
      国立高等専門学校機構
    • Industrial Property Rights Holder
      国立高等専門学校機構
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2015-198366
    • Filing Date
      2015-10-06
    • Related Report
      2015 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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