Development of analysis technique for accelerating protein aggregation and crystallization under applied electric fields and the clarification of the mechanism
Project/Area Number |
26390049
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Applied materials
|
Research Institution | Fukushima National College of Technology (2016) Ibaraki National College of Technology (2014-2015) |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
千葉 薫 茨城工業高等専門学校, 自然科学科, 准教授 (50415775)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
|
Keywords | タンパク質 / 凝集体 / 結晶化 / 光散乱 / 電場刺激 / フラクタル / 微粒子 |
Outline of Final Research Achievements |
We have developed a new analysis apparatus with the ability to in situ measure forward scattering light at small angle for crystallizing protein solutions under a low applied voltage. We tracked protein lysozyme aggregation in the pre-crystalline solutions using the developed apparatus. An enhancement of forward scattering intensities was observed for lysozyme solutions with a low-concentration salt under electric fields, which were under not crystallizing condition without applied voltages. We have found that the application of electric fields provides an effect of accelerating a formation of fractal aggregates on the pre-crystalline step.
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Report
(4 results)
Research Products
(16 results)