| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
In this research, we developed a quantitative optical path length difference and polarization microscopy (QPPM) and a digital holographic microscope (DHM). The QPPM was accomplished by introducing a polarimetric module to one arm of the Mach-Zehnder interferometer in our conventional phase imaging system. The QPPM can almost simultaneously measure the optical path length difference, birefringence phase difference, and the azimuth angle of birefringence, using our new base-correction method, which reduced the influence of elements other than cells. We also demonstrated the high-precision three-dimensional microscopic imaging of three-dimensionally arranged beads and living cells using our developed DHM. This DHM was completed by introducing a design method for an optical system that accurately acquires intensity distribution and phase distribution, and by improving the phase shift method and the three-dimensional data correction method.
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