| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
The use of a molecular beacon for rapid detection of DNA strand breaks induced by atmospheric pressure plasma jet (APPJ) irradiation was demonstrated. MBs are oligonucleotides that adopt a stem-and-loop structure and carry a 5’-fluorescent moiety and a 3’-nonfluorescent quenching moiety. Scission of the molecular beacon by APPJ irradiation leads to separation of the fluorophore-quencher pair, resulting in an increase in fluorescence that directly correlates with the DNA strand breaks. The results show that the increase in fluorescence intensity is proportional to the exposure time and the rate of fluorescence increase is proportional to the discharge power. In addition, This simple and rapid method allows the estimation of the extent of DNA stand breaks induced by exposure to a non-thermal plasma. In addition, it was shown that the plasma jet readily induced DNA-strand breaks in the cell model, surprisingly without any significant poration or rupture of the phospholipid membrane.
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