Analysis of DNA strand breaks induced by exposure to an atmospheric pressure plasma jet
| Project/Area Number |
26390096
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plasma electronics
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| Research Institution | Toyohashi University of Technology |
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Principal Investigator |
Kurita Hirofumi 豊橋技術科学大学, 工学(系)研究科(研究院), 助教 (70512177)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | プラズマ医療応用 / 大気圧低温プラズマ / DNA損傷 / DNA切断 / プラズマ医療 / 酸化ストレス / 大気圧プラズマ |
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| Outline of Final Research Achievements |
The use of a molecular beacon for rapid detection of DNA strand breaks induced by atmospheric pressure plasma jet (APPJ) irradiation was demonstrated. MBs are oligonucleotides that adopt a stem-and-loop structure and carry a 5’-fluorescent moiety and a 3’-nonfluorescent quenching moiety. Scission of the molecular beacon by APPJ irradiation leads to separation of the fluorophore-quencher pair, resulting in an increase in fluorescence that directly correlates with the DNA strand breaks. The results show that the increase in fluorescence intensity is proportional to the exposure time and the rate of fluorescence increase is proportional to the discharge power. In addition, This simple and rapid method allows the estimation of the extent of DNA stand breaks induced by exposure to a non-thermal plasma. In addition, it was shown that the plasma jet readily induced DNA-strand breaks in the cell model, surprisingly without any significant poration or rupture of the phospholipid membrane.
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Report
(4 results)
Research Products
(49 results)
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[Journal Article] Direct single-molecule observations of DNA unwinding by SV40 large tumor antigen under a negative DNA supercoil state.2018
Author(s)
Takahashi S, Motooka S, Kawasaki S, Kurita H, Mizuno T, Matsuura SI, Hanaoka F, Mizuno A, Oshige M, Katsura S
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Journal Title
Journal of Biomolecular Structure and Dynamics,
Volume: 5
Pages: 1-13
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Pore formation in artificial cell membranes induced by atmospheric pressure plasma2017
Author(s)
Ryugo Tero, Kota Yusa, Kyohei Fukuda, Yoshiyuki Suda, Hachiro Yasuda, Hirofumi Kurita, Kazunori Takashima, Akira Mizuno, Yuu Hirose, Toru Harigai, Hirofumi Takikawa
Organizer
International Conference on Plasma Medical Science Innovation (ICPMSI) 2017
Place of Presentation
名古屋大学
Year and Date
2017-02-26
Related Report
Int'l Joint Research
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[Presentation] Endre Szili, Sung-Ha Hong, Nishtha Gaur, Jun-Seok Oh, Masafumi Ito, Akimitsu Hatta, Hiroshi Furuta, Hirofumi Kurita, Akira Mizuno, Rob Short2017
Author(s)
Endre Szili, Sung-Ha Hong, Nishtha Gaur, Jun-Seok Oh, Masafumi Ito, Akimitsu Hatta, Hiroshi Furuta, Hirofumi Kurita, Akira Mizuno, Rob Short
Organizer
International Conference on Plasma Medical Science Innovation (ICPMSI) 2017
Place of Presentation
名古屋大学
Year and Date
2017-02-26
Related Report
Int'l Joint Research
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[Presentation] Plasma-induced chemical and physical performation and its applications of gene injection2017
Author(s)
Yoko Yamanishi, Ryugo Tero, Satoshi Uchida, Kota Yusa, Kyohei Fukuda, Yoshiyuki Suda, Hachiro Yasuda, Hirofumi Kurita, Kazunori Takashima, Akira Mizuno, Yuu Hirose, Toru Harigai, Hirofumi Takikawa
Organizer
International Conference on Plasma Medical Science Innovation (ICPMSI) 2017
Place of Presentation
名古屋大学
Year and Date
2017-02-26
Related Report
Int'l Joint Research
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