| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
Complicated conformations of DNA appear when strain is accumulated by stretching and twisting DNA. In living cells, gene expression is regulated by regulating the higher order conformation of DNA. In this study, we have developed a novel tweezer which enables us to stretch and twist a single DNA independently, and succeeded to observe the conformational change in low force regime that is hard to access by traditional method. Our results indicate that not only a protein design but also conformational design can be coded in DNA base sequence.
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