Structural Study of protein A and its derivatives in solution

• Project/Area Number: 26400438
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biological physics/Chemical physics/Soft matter physics
• Research Institution: Himeji Hinomoto College
• Principal Investigator: Kihara Hiroshi 姫路日ノ本短期大学, その他部局等, 特任教授 (20049076)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2016: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000); Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: ｘ線溶液散乱 / プロテインA / 蛋白質の溶液内構造 / 免疫グロブリン / protein A / X線溶液散乱法 / IgG の Fc fragment / 免疫抗体 / 溶液内構造 / 免疫グロブリンG

Outline of Final Research Achievements

Protein A is oftein used for preparation of immunoglobulins. However, its conformation is solution has not known. We have used X-ray solution scattering method for obtaining the conformation of protein A and its derivatives.
Results are also compared with the structure predicted by molecular dynamics, which gives the very good coincidence. This result suggests the protein A takes the relaxed conformaton without the stress.
We have also done X-ray solution scattering study on the complex of protein A and Fc fragment of Immunoglobulin, and successfully obtained the scattering profile of the complex. Further analysis should be done.

Research Products

• [Journal Article] A redox switch shapes the Lon protease exit pore to faclutatively regulate proteolysis (2015)
  • Author(s): Nishi, W., Kukimoto-Niino, M., Terada, T., Shirouzu, M., Muramatsu, T., Kojima, M., Kihara, H., and Yokoyama, S.
  • Journal Title: Nat. Chem. Biol. Volume: 11 Issue: 1 Pages: 46-51
  • DOI: 10.1038/nchembio.1688
  • Peer Reviewed / Open Access
• [Journal Article] A Physicochemical and mutational analysis of intersubunit interactions of Eshcerichia coli ferritin A. (2015)
  • Author(s): Ohtomo, H., Ohtomo, M. sato, D., Kurobe, A., Sunato, A., Matsumura, Y., Kihara, H., Fujiwara, K. Ikeguchi, M.
  • Journal Title: Biochemistry Volume: 54 Issue: 40 Pages: 6243-6251
  • DOI: 10.1021/acs.biochem.5b00723
  • Peer Reviewed / Open Access
• [Journal Article] A redox switch shapes the Lon protease exit pore to facultively regulate proteolysis (2015)
  • Author(s): Nishii, W., Kukimoto-Niino, M., Terada, T., Shirouzu, M., Muramatsu, T., Kojima, M., Kihara, H., Yokoyama, S.
  • Journal Title: Nature Chemical Biology Volume: 11 Pages: 46-51
  • Peer Reviewed
• [Journal Article] Structural stability of E. coli trigger factor studied by synchrotron small-angle X-ray scattering, ., (2014)
  • Author(s): Yi Shi, Masaji Shinjo, Jun-Mei Zhou, Hiroshi Kihara
  • Journal Title: Biophys. Chem Volume: 175-176 Pages: 39-46
  • Peer Reviewed
• [Journal Article] C-terminal 13-residue truncation in-duces compact Trigger Factor conformation and severely impairs its dimerization ability, (2014)
  • Author(s): Yi Shi, Ling Yu, Hiroshi Kihara and Jun-Mei Zhou
  • Journal Title: Protein & Peptide Letters Volume: 21 Pages: 476-482
  • Peer Reviewed
• [Journal Article] alpha-helix formation rate of loigopeptides at subzero temperatures (2014)
  • Author(s): Zhi-jie Qin, Akio Shimizu, Jinsong Li, Masamichi Ikeguchi, Masaji Shinjo and Hiroshi Kihara
  • Journal Title: Biophysics Volume: 10 Pages: 9-13
  • Peer Reviewed / Open Access
• [Presentation] Expansion of Whack-A mole model to a time-lag phase of radiation effect (2015)
  • Author(s): Hiroshi Kihara
  • Organizer: YITP International Workshop: Biological & Medical Science based on Physics:
  • Place of Presentation: 京都大学湯川研究所
  • Year and Date: 2015-11-05
• [Presentation] 放射光を光源とした軟X線顕微鏡によるカビ臭産生シアノバクテリアの観察 (2015)
  • Author(s): 竹本邦子，吉村真史，難波秀利，木原 裕
  • Organizer: 第５６回日本組織細胞化学会
  • Place of Presentation: 関西医科大学
  • Year and Date: 2015-10-03
• [Presentation] Sirt4 複合体構造モデリング (2015)
  • Author(s): 小島 正樹
  • Organizer: 日本バイオイメージング学会
  • Place of Presentation: 東京理科大学葛飾キャンパス
  • Year and Date: 2015-09-26
• [Presentation] Protein A 及びその誘導体のX線溶液散乱法による構造解析 (2015)
  • Author(s): 新庄正路，市村 薫，山本章嗣, 木原 裕
  • Organizer: 第５３回日本生物物理学会
  • Place of Presentation: 金沢大学角間キャンパス
  • Year and Date: 2015-09-13
• [Presentation] Lon protease from Enterobacteriaceal facultative anaerobes is a redox-switched AAA+ protease (2014)
  • Author(s): Wataru Nishii, Mutsuko Kukimoto-Niino, Takaho Terada, Mikako Shirouzu, Tomonari Muramatsu Masaki Kojima, Hiroshi Kihara Shigeyuki Yokoyama
  • Organizer: 日本生化学会
  • Place of Presentation: 国立京都会館
  • Year and Date: 2014-10-15 – 2014-10-18