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Establishment of novel environmental proxies based on synthetical analysis for DNA, isotopes, and morphology of planktonic foraminiferal single-cell

Research Project

Project/Area Number 26400501
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Stratigraphy/Paleontology
Research InstitutionKochi University (2016)
Shinshu University (2014-2015)

Principal Investigator

Ujiie Yurika  高知大学, 教育研究部自然科学系理学部門, 准教授 (20573041)

Co-Investigator(Kenkyū-buntansha) 木元 克典  国立研究開発法人海洋研究開発機構, 地球環境観測研究開発センター, 主任技術研究員 (40359162)
石村 豊穂  茨城工業高等専門学校, 物質工学科, 准教授 (80422012)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords海洋環境指標 / 浮遊性有孔虫 / 分子系統解析 / 形態分析 / 安定同位体比 / 遺伝子型 / 非破壊形態分析 / 酸素・炭素安定同位体 / 共生藻 / 炭素循環 / 遺伝子解析 / 安定酸素・炭素同位体比 / 同位体比分析
Outline of Final Research Achievements

Planktonic foraminifers are the most useful microfossil as ocean environmental proxy. However, the previous biological studies have pointed out that their taxonomic complexity veil the true ecological trait of each biological species. This study aimed to develop and apply the new approach, which comprehensively conduct the molecular, morphometric, and geochemical analyses, on an individual of planktonic foraminifers. First, we precisely examined the DNA extraction method has no physical or chemical damages to the foraminiferal shells, by using the leading-edge technologies. Then, we measured the morphological characters of the shells, and oxygen and carbon stable isotopes for each individual. Finally, we succeeded to detect morphological and isotopic differences between different biological species. Our novel approach is available for broad fossilized organisms to advance the understanding of their ecological characters, which could be worked for development of new multiple proxies.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (27 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (3 results) Journal Article (9 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 9 results,  Open Access: 3 results,  Acknowledgement Compliant: 3 results) Presentation (15 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results)

  • [Int'l Joint Research] Station Biologique de Roscoff/University of Lyon/CEREGE(フランス)

    • Related Report
      2015 Research-status Report
  • [Int'l Joint Research] University of Bremen(ドイツ)

    • Related Report
      2015 Research-status Report
  • [Int'l Joint Research] University of Edinburgh/University of St Andrews/University of Nottingham(英国)

    • Related Report
      2015 Research-status Report
  • [Journal Article] Nomenclature for the Nameless: a proposal for an integrative Molecular taxonomy of Cryptic Diversity Exemplified by Planktonic Foraminifera2016

    • Author(s)
      R.M. Morard, G. Escarguel, A. K.M. Weiner, A. Andre, C.J. Douady, C. M. Wade, K. Darling, Y. Ujiie, H.A. Seears, and F. Quillevere T.de Garidel,- Thoron, C. de Vargas and M. Kucera
    • Journal Title

      Systematic Biology

      Volume: 10 Issue: 5 Pages: 1-31

    • DOI

      10.1093/sysbio/syw031

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Evolution of the North Pacific Subtropical Gyre during the past 190kyr through the interaction of the Kuroshio Current with the surface and intermediate waters2016

    • Author(s)
      Yurika Ujiie, Hirofumi Asahi, Takuya Sagawa, and Franck Bassinot
    • Journal Title

      Paleoceanography

      Volume: - Issue: 11 Pages: 1498-1513

    • DOI

      10.1002/2015pa002914

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Methodology for single-cell genetic analysis of planktonic foraminifera for studies of protist ecology and evolution2016

    • Author(s)
      Weiner AKM, Morard R, Weinkauf MFG, Darling KF, Andre A, Quillevere; F, Ujiie Y, Douady CJ, de Vargas C, Kucera M
    • Journal Title

      Frontiers in Marine Science

      Volume: 3 Pages: 255-255

    • DOI

      10.3389/fmars.2016.00255

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Evolution of a Planktonic Foraminifer during Environmental Changes in the Tropical Oceans.2016

    • Author(s)
      Ujiie, Y. and Y. Ishitani
    • Journal Title

      PlosOne

      Volume: 11 Issue: 2 Pages: e0148847-e0148847

    • DOI

      10.1371/journal.pone.0148847

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] マイクロX線CTで取得した3次元形状情報に基づく有孔虫骨格モデルのパラメータ同定2016

    • Author(s)
      岸本直子・木元克典
    • Journal Title

      化石

      Volume: 99 Pages: 53-53

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] 計算微古生物学:最 新3次元イメージング技術の浮遊性有孔虫研究への応用2016

    • Author(s)
      佐々木理, 岩下智洋, 木元克典, 鹿納晴尚, 木原辰之
    • Journal Title

      化石

      Volume: 99 Pages: 63-63

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] PFR2: a curated database of planktonic foraminifera 18S ribosomal DNA as a resource for studies of plankton ecology, biogeography and evolution.2015

    • Author(s)
      Morard, R., K.F. Darling, F. Mahe, S. Audic, Y. Ujiie, A.K.M. Weiner, A. Andre, H.A. Seears, C. Wade, F. Quillevere, C.J. Douady, G. Escarguel, T. de Garidel-Thoron, M. Siccha, M. Kucera, and C. de Vargas
    • Journal Title

      Molecular Ecology Resources

      Volume: 15 Issue: 6 Pages: 1472-1472

    • DOI

      10.1111/1755-0998.12410

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Exploring photosymbiotic ecology of planktic foraminifers from chamber-by-chamber isotopic history of individual foraminifers2015

    • Author(s)
      Haruka Takagi, Kazuyoshi Moriya, Toyoho Ishimura, Atsushi Suzuki, Hodaka Kawahata, Hiromichi Hirano
    • Journal Title

      Paleobiology

      Volume: 41 Issue: 1 Pages: 108-121

    • DOI

      10.1017/pab.2014.7

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Uncovering sibling species in Radiolaria: evidence for ecological partitioning in a marine planktonic protist2014

    • Author(s)
      Yoshiyuki Ishitani, Yurika Ujiie, Kiyotaka Takishita
    • Journal Title

      Molecular Phylogenetics and Evolution

      Volume: 78 Pages: 215-222

    • DOI

      10.1016/j.ympev.2014.05.021

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 微化石研究におけるバイオメトリックス:マイクロX線CTの活用2017

    • Author(s)
      木元克典、佐々木理、岸本直子
    • Organizer
      MRC微化石研究集会山形大会
    • Place of Presentation
      山形大学
    • Year and Date
      2017-03-02
    • Related Report
      2016 Annual Research Report
  • [Presentation] 浮遊性1個体からわかる遺伝子・形態・同位体-3要素同時解析の実現-2017

    • Author(s)
      氏家由利香、木元克典、石村豊穂
    • Organizer
      日本古生物学会第166回例会
    • Place of Presentation
      早稲田大学
    • Year and Date
      2017-01-29
    • Related Report
      2016 Annual Research Report
  • [Presentation] 生物源炭酸塩に記録された生態履歴の高解像度抽出への挑戦:魚類耳石を例として2017

    • Author(s)
      石村豊穂
    • Organizer
      日本古生物学会第166回例会・シンポジウム
    • Place of Presentation
      早稲田大学
    • Year and Date
      2017-01-27
    • Related Report
      2016 Annual Research Report
  • [Presentation] 有孔虫安定同位体組成のバラツキを活かす:海洋環境指標としての信頼性評価とアーカイブ試料の活用2016

    • Author(s)
      石村豊穂・長谷川四郎・池原研
    • Organizer
      日本地球化学会
    • Place of Presentation
      大阪市立大学
    • Year and Date
      2016-09-13
    • Related Report
      2016 Annual Research Report
  • [Presentation] 環境勾配の発達による熱帯・外洋域のプランクトンの進化2016

    • Author(s)
      氏家由利香、石谷 佳之
    • Organizer
      日本進化学会第18回大会
    • Place of Presentation
      東京工業大学
    • Year and Date
      2016-08-26
    • Related Report
      2016 Annual Research Report
  • [Presentation] マイクロXCTで取得した3次元形状情報に基づく浮遊性有孔虫骨格モデルのパラメータ同定2016

    • Author(s)
      岸本直子、木元克典
    • Organizer
      日本地球惑星科学連合2016年大会
    • Place of Presentation
      幕張メッセ
    • Year and Date
      2016-05-23
    • Related Report
      2016 Annual Research Report
  • [Presentation] Approach to comprehensive analyses of molecular phylogeny, morphometrics, and geochemistry of planktonic foraminifera2016

    • Author(s)
      Yurika Ujiie, Katsunori Kimoto, Toyoho Ishimura, and Naoko Kishimoto
    • Organizer
      日本地球惑星科学連合2016年大会
    • Place of Presentation
      幕張メッセ
    • Year and Date
      2016-05-22
    • Related Report
      2016 Annual Research Report
  • [Presentation] マイク ロX線CT法による生物骨格の3次元計測2015

    • Author(s)
      木元 克典,中村 由里子, 佐々木 理,鹿納 晴尚,岩崎 晋也
    • Organizer
      日本サンゴ礁学会
    • Place of Presentation
      慶応大学三田キャンパス・東京
    • Year and Date
      2015-11-26
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] 化石記録と分子時計からみた浮遊性有孔虫の進化2015

    • Author(s)
      氏家由利香, 石谷佳之
    • Organizer
      日本古生物学会2015年年会
    • Place of Presentation
      産業技術総合研究所・筑波
    • Year and Date
      2015-06-28
    • Related Report
      2015 Research-status Report
  • [Presentation] Micro focus X-ray Computing Tomography Technique: Applications for the Ocean Acidification study2015

    • Author(s)
      Kimoto, K., Kihara, T., Iwasaki, S., Sasaki, O., Kano, H., Wakita, M., Irino, T.
    • Organizer
      日本地球惑星科学連合 2015年大会
    • Place of Presentation
      幕張メッセ・千葉
    • Year and Date
      2015-05-28
    • Related Report
      2015 Research-status Report
  • [Presentation] Seasonal variation in genetic types of a planktonic foraminifer2015

    • Author(s)
      Ujiie, Y. and H. Ueda
    • Organizer
      日本地球惑星科学連合 2015年大会
    • Place of Presentation
      幕張メッセ・千葉
    • Year and Date
      2015-05-26
    • Related Report
      2015 Research-status Report
  • [Presentation] 安定同位体比質量分析計IsoPrime100を用いた高精度・高感度分析システムの開発2015

    • Author(s)
      中根雅晴・田中崇史・鐵智美・高木悠花・石村豊穂
    • Organizer
      日本地球惑星科学連合 2015年大会
    • Place of Presentation
      幕張メッセ・千葉
    • Year and Date
      2015-05-24
    • Related Report
      2015 Research-status Report
  • [Presentation] CT analysis reveals shell density of marine calcifiers and its seasonal changes2015

    • Author(s)
      Kimoto, K
    • Organizer
      国際北極研究シンポジウム
    • Place of Presentation
      富山国際会場・富山
    • Year and Date
      2015-04-27
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] How do the unicellular zooplankton diverge in pelagic ocean?2014

    • Author(s)
      Ujiie, Y. and Y. Ishitani
    • Organizer
      World Conference on Marine Biodiveristy
    • Place of Presentation
      中国・チンタオ
    • Year and Date
      2014-10-13
    • Related Report
      2014 Research-status Report
  • [Presentation] 微小領域における炭酸塩の安定同位体比分析の現状2014

    • Author(s)
      石村豊穂
    • Organizer
      日本地球化学会年会
    • Place of Presentation
      富山大学
    • Year and Date
      2014-09-16
    • Related Report
      2014 Research-status Report
    • Invited

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Published: 2014-04-04   Modified: 2022-02-03  

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