| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Outline of Final Research Achievements |
We studied photochemically induced phase transition of lipid to obtain membrane protein crystal. Crystallization of membrane proteins is mainly performed by using lipid phase transition as a driving force. In this study, we investigated phase transition of lipid with the use of a photochemical surfactant whose structure is changed by trans-cis isomerization. AZTMA having an azobenzene group was prepared as a photofunctional surfactant. As a result, it was confirmed that the phase transition of lipid phase from cubic phase to lamellar phase induced by trans-cis isomerization reaction of AZTMA by polarization microscopy and X-ray diffraction experiment. Crystallization experiments were carried out using bacteriorhodopsin as a membrane protein. The presence of the membrane protein molecule acted as a driving force causing a phase transition, and crystallization did not observed at the photo phase transition.
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