Photoinduced phase transition of lipid / water / photo-functional surfactant system to obtain membrane protein crystals.
Project/Area Number |
26410003
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Physical chemistry
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Research Institution | Gunma University |
Principal Investigator |
OKUTSU TETSUO 群馬大学, 大学院理工学府, 教授 (20261860)
|
Co-Investigator(Kenkyū-buntansha) |
堀内 宏明 群馬大学, 大学院理工学府, 准教授 (00334136)
高橋 浩 群馬大学, 大学院理工学府, 教授 (80236314)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
Keywords | 結晶成長 / タンパク質 / 光誘起結晶化 / 膜タンパク質 / 光誘起相転移 / 結晶化 / 光異性化 / 相転移 / 脂質 / 膜タンパク質の結晶化 / バクテリオロドプシン / 脂質相転移 / 光結晶化 / モノオレイン |
Outline of Final Research Achievements |
We studied photochemically induced phase transition of lipid to obtain membrane protein crystal. Crystallization of membrane proteins is mainly performed by using lipid phase transition as a driving force. In this study, we investigated phase transition of lipid with the use of a photochemical surfactant whose structure is changed by trans-cis isomerization. AZTMA having an azobenzene group was prepared as a photofunctional surfactant. As a result, it was confirmed that the phase transition of lipid phase from cubic phase to lamellar phase induced by trans-cis isomerization reaction of AZTMA by polarization microscopy and X-ray diffraction experiment. Crystallization experiments were carried out using bacteriorhodopsin as a membrane protein. The presence of the membrane protein molecule acted as a driving force causing a phase transition, and crystallization did not observed at the photo phase transition.
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Report
(4 results)
Research Products
(50 results)
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[Journal Article] Extension of pai-System of Silylated Porphyrin Derivative for Photodynamic Therapy2016
Author(s)
Hiroaki Horiuchi, Asumi Sakai, Shingo Akiyama, Remi Ikeda, Satoshi Ito, Masaru Furuya, Yuta Gomibuchi, Muneki Ichikawa, Toshitada Yoshihara, Seiji Tobita, Tetsuo Okutsu
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Journal Title
Journal of Photochemistry and Photobiology A: Chemistry
Volume: 339
Pages: 19-24
DOI
Related Report
Peer Reviewed
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