Applications of Near-Infrared Raman Optical Activity to Photoreceptor Proteins

• Project/Area Number: 26410017
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Physical chemistry
• Research Institution: Saga University
• Principal Investigator: Unno Masashi 佐賀大学, 工学(系)研究科(研究院), 教授 (50255428)
• Co-Investigator(Renkei-kenkyūsha): MASUDA Shinji 東京工業大学, バイオ研究基盤支援総合センター, 准教授 (30373369) ; TAMOGAMI Jun 松山大学, 薬学部, 助教 (30580089) ; KOMATSU Naoki 滋賀医科大学, 医学部, 准教授 (30253008)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 振動分光 / 生物物理 / 光受容タンパク質 / 量子化学計算 / 分子分光学 / 生物物理学 / 国際研究者交流 / 国際研究者交流（米国）

Outline of Final Research Achievements

Many biological cofactors, such as light-absorbing chromophores in photoreceptors, are intrinsically planar molecules. A protein environment, however, causes structural distortions of the cofactor, and such structural changes can lead to a modulation of chemical properties of the cofactor to maximize its biological activity. In this project, we have established the spectral analysis of Raman optical activity (ROA) based on quantum chemical calculations combined with molecular dynamics simulations. For an application of ROA to photoreceptor proteins, we have examined the effect of excitation wavelength on the ROA spectra for the first time.

Research Products

• [Int'l Joint Research] Oklahoma State University/Michigan State University(米国)
• [Int'l Joint Research] RMIT University(オーストラリア)
• [Int'l Joint Research] Oklahoma State University(米国)
• [Journal Article] Raman optical activity of tetra-alanine in the poly(L-proline) II type peptide conformation (2017)
  • Author(s): Masakazu Furuta, Tomotsumi Fujisawa, Hiroyasu Urago, Takahiro Eguchi, Takahito Shingae, Satoshi Takahashi, Ewan W. Blanch, Masashi Unno
  • Journal Title: Phys. Chem. Chem. Phys. Volume: 19 Issue: 3 Pages: 2078-2086
  • DOI: 10.1039/c6cp07828a
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] ラマン光学活性分光法 (2016)
  • Author(s): 藤澤知績、海野雅司
  • Journal Title: 分光研究 Volume: 65 Pages: 218-231
  • NAID: 40020995115
  • Peer Reviewed
• [Journal Article] ラマン分光法の有田焼評価への活用 (2016)
  • Author(s): 松尾英之、海野雅司
  • Journal Title: セラミックス Volume: 51 Pages: 550-552
• [Journal Article] Experimental detection of the intrinsic difference in Raman optical activity of a photoreceptor protein under pre-resonance and resonance conditions (2015)
  • Author(s): Haraguchi, S., Hara, M., Shingae, T., Kumauchi, M., Hoff, W. D., Unno, M.
  • Journal Title: Angew. Chem. Int. Ed. Volume: 54 Issue: 39 Pages: 11555-11558
  • DOI: 10.1002/anie.201505466
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The Roles of C-terminal Residues on the Thermal Stability and Local Heme Environment of Cytochrome c' from the Thermophilic Purple Sulfur Bacterium Thermochromatium (2015)
  • Author(s): Kimura, Y., Kasuga, S., Unno, M., Furusawa, T., Osoegawa, S., Sasaki, Y., Ohno, T., and Wang-Otomo, Z.-Y.
  • Journal Title: Photosynthesis Research Volume: 124 Issue: 1 Pages: 19-29
  • DOI: 10.1007/s11120-014-0069-6
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Influence of Preparation Temperature of Precursor on the Thermal Stability of TiO2(B) and its Electrochemical Property as an Anode Material in the Lithium-Ion Battery (2014)
  • Author(s): Furuya, Y., Zhao, W., Iida, T., Unno, M., & Noguchi, H.
  • Journal Title: Electrochemistry Volume: 82 Pages: 7-13
  • NAID: 130003387730
  • Peer Reviewed / Open Access
• [Journal Article] The Electrochemical Properties of Low-crystallinity TiO2(B)-Carbon Composite as an Anode Material in Lithium Ion Battery (2014)
  • Author(s): Furuya, Y., Zhao, W., Unno, M., & Noguchi, H.
  • Journal Title: Electrochim. Acta Volume: 136 Pages: 266-273
  • DOI: 10.1016/j.electacta.2014.05.107
  • Peer Reviewed
• [Journal Article] Raman Optical Activity of a Cyclic Dipeptide Analyzed by Quantum Chemical Calculations Combined with Molecular Dynamics Simulations (2014)
  • Author(s): Urago, H., Suga, T., Hirata, T., Kodama, H., & Unno, M.
  • Journal Title: J. Phys. Chem. B Volume: 118 Issue: 24 Pages: 6767-6774
  • DOI: 10.1021/jp503874z
  • Peer Reviewed
• [Journal Article] Synthesis and characterization of anisotropically expanded graphite oxide compounds derived from spherical graphite (2014)
  • Author(s): Zhao, W., Kido, G., Harada, S. Unno, M., Noguchi, H.
  • Journal Title: J. Colloid Interface Sci. Volume: 431 Pages: 8-16
  • DOI: 10.1016/j.jcis.2014.06.018
  • Peer Reviewed
• [Journal Article] Synthesis of LiNi1/3Mn2/3O2 related cathode material from P2-Na2/3Ni1/3Mn2/3O2 for lithium ion battery (2014)
  • Author(s): Zhao, W., Tanaka, A., Tanaka, A., Unno, M., Yamamoto, S., Noguchi, H.
  • Journal Title: Mater. Lett. Volume: 134 Pages: 206-209
  • DOI: 10.1016/j.matlet.2014.07.042
  • Peer Reviewed
• [Journal Article] Synthesis of metal ion substituted P2-Na2/3Ni1/3Mn2/3O2 cathode material with enhanced performance for Na ion batteries (2014)
  • Author(s): Zhao, W., Kirie, H., Tanaka, A., Unno, M., Yamamoto, S., Noguchi, H.
  • Journal Title: Mater. Lett. Volume: 135 Pages: 131-134
  • DOI: 10.1016/j.matlet.2014.07.153
  • Peer Reviewed
• [Presentation] 紫外共鳴ラマン分光法を用いたシアノバクテリオクロムRcaEの光変換機構の解明 (2017)
  • Author(s): 渡邉 亘平、小副川 晋介、広瀬 侑、藤澤 知績、池内 昌彦、海野 雅司
  • Organizer: 日本化学会 第97春季年会 (2017)
  • Place of Presentation: 横浜
  • Year and Date: 2017-03-16
• [Presentation] Assignment of Raman Optical Activity Spectra of Photoactive Yellow Protein by Isotopic Labeling (2017)
  • Author(s): HARAGUCHI, Shojiro; SHINGAE, Takahito; KASAI, Noritaka; KUMAUCHI, Masato; FUJISAWA, Tomotsumi; HANAMOTO, Takeshi; HOFF, Wouter D.; UNNO, Masashi
  • Organizer: 日本化学会 第97春季年会 (2017)
  • Place of Presentation: 横浜
  • Year and Date: 2017-03-16
• [Presentation] Searching for anhamonic low frequency mode in green fluorescent protein (2017)
  • Author(s): FUJISAWA, Tomotsumi; UNNO, Masashi
  • Organizer: 日本化学会 第97春季年会 (2017)
  • Place of Presentation: 横浜
  • Year and Date: 2017-03-16
• [Presentation] Chromophore conformation in active site of orange carotenoid protein from Raman optical activity spectroscopy (2016)
  • Author(s): Tomotsumi Fujisawa、Masashi Unno、Ryan L. Leverenz、Cheryl A. Kerfeld
  • Organizer: 第54回日本生物物理学会年会
  • Place of Presentation: つくば
  • Year and Date: 2016-11-25
• [Presentation] 同位体標識試料を用いた BLUF ドメインの水素結合環境の解明 (2016)
  • Author(s): Takashi Nagai, Tatsuya Iwata, Shota Ito, Mineo Iseki, Masakatsu Watanabe, Masashi Unno, Shinya Kitagawa, Hideki Kandori
  • Organizer: 第54回日本生物物理学会年会
  • Place of Presentation: つくば
  • Year and Date: 2016-11-25
• [Presentation] Raman Optical Activity of Photoreceptor Proteins under Pre-resonance and Resonance Conditions (2016)
  • Author(s): Masashi Unno
  • Organizer: Fifth International Conference on Vibrational Optical Activity
  • Place of Presentation: Antwerp, Belgium
  • Year and Date: 2016-09-11
  • Int'l Joint Research / Invited
• [Presentation] Chromophore conformation in active site of orange carotenoid protein from Raman optical activity (2016)
  • Author(s): Tomotsumi Fujisawa、Ryan L. Leverenz、Cheryl A. Kerfeld、Masashi Unno
  • Organizer: Fifth International Conference on Vibrational Optical Activity
  • Place of Presentation: Antwerp, Belgium
  • Year and Date: 2016-09-11
  • Int'l Joint Research
• [Presentation] Photoactive Yellow Protein発色団の面外方向への歪みに敏感なC-H面外変角振動モードの帰属 (2016)
  • Author(s): 原口 翔次郎、新ヶ江 貴仁、笠井 紀貴、熊内 雅人、藤澤 知績、花本 猛士、Wouter D. Hoff、海野 雅司
  • Organizer: 九重分子科学セミナー2016
  • Place of Presentation: 大分
  • Year and Date: 2016-09-02
• [Presentation] 紫外共鳴ラマン分光法によるシアノバクテリオクロムRcaEの光変換機構の解析 (2016)
  • Author(s): 渡邉 亘平、小副川 晋介、広瀬 侑、藤澤 知績、池内 昌彦、海野 雅司
  • Organizer: 九重分子科学セミナー2016
  • Place of Presentation: 大分
  • Year and Date: 2016-09-02
• [Presentation] ラマン光学活性に基づくオレンジカロテノイドタンパク質の活性部位における発色団の立体構造変化 (2016)
  • Author(s): 藤澤知績、Ryan L. Leverenz、Cheryl A. Kerfeld、海野雅司
  • Organizer: 第 43 回 生体分子科学討論会
  • Place of Presentation: 名古屋
  • Year and Date: 2016-06-24
• [Presentation] Experimental detection of the intrinsic difference in Raman optical activity of a photoreceptor protein under pre-resonance and resonance conditions (2016)
  • Author(s): Haraguchi, S., Hara, M., Shingae, T., Kumauchi, M., Hoff, W. D., Unno, M.
  • Organizer: 日本化学会 第96春季年会
  • Place of Presentation: 京都
  • Year and Date: 2016-03-27
• [Presentation] Chromophore conformation in active site of orange carotenoid protein studied by Raman optical activity spectroscopy (2016)
  • Author(s): FUJISAWA, Tomotsumi; LEVERENZ, Ryan; KERFELD, Cheryl; UNNO, Masashi
  • Organizer: 日本化学会 第96春季年会
  • Place of Presentation: 京都
  • Year and Date: 2016-03-25
• [Presentation] ラマン分光法の有田焼への活用について (2016)
  • Author(s): 渡孝則、海野雅司、嘉村翔太郎、松尾英之
  • Organizer: 第54回セラミックス基礎科学討論会
  • Place of Presentation: 佐賀
  • Year and Date: 2016-01-07
• [Presentation] ラマン円偏光二色性分光を用いた色素タンパク質の構造解析 (2016)
  • Author(s): 海野雅司
  • Organizer: 第四回 分子設計研究会
  • Place of Presentation: 東京
  • Invited
• [Presentation] Experimental detection of the intrinsic difference in Raman optical activity of a photoreceptor protein under pre-resonance and resonance conditions (2015)
  • Author(s): Haraguchi, S., Hara, M., Shingae, T., Kumauchi, M., Hoff, W. D., Unno, M.
  • Organizer: The 6th Joint Seminar between Saga University and Liaoning University
  • Place of Presentation: 佐賀
  • Year and Date: 2015-12-10
  • Int'l Joint Research
• [Presentation] Evidence for the presence of K and L intermediates in proteorhodopsin revealed by transient resonance Raman spectroscopy (2015)
  • Author(s): Yamaryo, H., Tajitsu, S., Tamogami, J., Fujisawa, T., Kamo, N., Unno, M.
  • Organizer: The 6th Joint Seminar between Saga University and Liaoning University
  • Place of Presentation: 佐賀
  • Year and Date: 2015-12-10
  • Int'l Joint Research
• [Presentation] Experimental detection of the intrinsic difference in Raman optical activity of a photoreceptor protein under pre-resonance and resonance conditions (2015)
  • Author(s): Haraguchi, S., Hara, M., Shingae, T., Kumauchi, M., Hoff, W. D., Unno, M.
  • Organizer: 12th Annual Research Symposium in Biological Sciences
  • Place of Presentation: Oklahoma
  • Year and Date: 2015-09-17
  • Int'l Joint Research
• [Presentation] 過渡共鳴ラマン分光法を用いたプロテオロドプシン光反応初期中間体の解析 (2015)
  • Author(s): 山領 春輝、田実 真一、田母神 淳、加茂 直樹、海野 雅司
  • Organizer: 第53回日本生物物理学会年会
  • Place of Presentation: 金沢
  • Year and Date: 2015-09-13
• [Presentation] Refinements of the Active Site Structures in Photoreceptor Proteins by Raman Optical Activity (2015)
  • Author(s): Masashi Unno
  • Organizer: 15th International Conference on Chiroptical Spectroscopy
  • Place of Presentation: Sapporo
  • Year and Date: 2015-08-30
  • Int'l Joint Research / Invited
• [Presentation] 過渡共鳴ラマン分光法を用いたプロテオロドプシン初期中間体の解析 (2015)
  • Author(s): 山領 春輝、田実 真一、田母神 淳、加茂 直樹、海野 雅司
  • Organizer: 九重分子科学セミナー2015
  • Place of Presentation: 九重
  • Year and Date: 2015-07-24
• [Presentation] Active Site Structures of Heme Proteins Studied by Raman Optical Activity (2015)
  • Author(s): T. Shingaea, M. Unno
  • Organizer: "Metals in Biology” in Wako
  • Place of Presentation: 和光
  • Year and Date: 2015-06-16
  • Int'l Joint Research
• [Presentation] Exploring the active site structure of photoreceptor proteins by Raman optical activity (2015)
  • Author(s): Masashi Unno
  • Organizer: APS March Meeting 2015
  • Place of Presentation: San Antonio, TX, USA
  • Year and Date: 2015-03-03
  • Invited
• [Presentation] Near-Infrared Raman Optical Activity Probes the Active Site Structure of Photoreceptor Proteins (2015)
  • Author(s): Masashi Unno
  • Organizer: Departmental Seminar, Department of Biochemistry & Molecular Biology, Oklahoma State University
  • Place of Presentation: Stillwater, OK, USA
  • Year and Date: 2015-02-27
  • Invited
• [Presentation] Active Site Structure of Photoactive Yellow protin with a Locked Chromophore Analoge Revealed by Near-Infrared Raman Optical Activity (2014)
  • Author(s): Takahito Shingae, Kensuke Kubota, Nicole D. Foster, Masato Kumauchi, Wouter D. Hoff, Masashi Unno
  • Organizer: The 9th Saga University-Daegu University Joint Seminar
  • Place of Presentation: 佐賀
  • Year and Date: 2014-11-17 – 2014-11-19
• [Presentation] Near-Infrared Raman Optical Activity of Photoactive Yellow Protein with a Locked Chromophore Analogue Analyzed by Quantum Chemical Calculations and Molecular Dynamics Simulations (2014)
  • Author(s): Shojiro Haraguchi, Takahito Shingae, Masato Kumauchi, Wouter D. Hoff, Masashi Unno
  • Organizer: The 9th Saga University-Daegu University Joint Seminar
  • Place of Presentation: 佐賀
  • Year and Date: 2014-11-17 – 2014-11-19
• [Presentation] 分子動力学計算と量子化学計算を用いた環状ジペプチドのラマン円偏光二色性スペクトルの解析 (2014)
  • Author(s): ○海野雅司，浦郷寛康，菅虎雄，兒玉浩明
  • Organizer: 第３７回溶液化学シンポジウム
  • Place of Presentation: 佐賀
  • Year and Date: 2014-11-12 – 2014-11-13
• [Presentation] 構造揺らぎを考慮して光受容タンパク質のラマン円偏光二色性スペクトルを解析する (2014)
  • Author(s): ○原口 翔次郎，古賀 菜月, 新ヶ江 貴仁, 熊内 雅人, Wouter D. HOFF, 海野 雅司
  • Organizer: 第３７回溶液化学シンポジウム
  • Place of Presentation: 佐賀
  • Year and Date: 2014-11-12 – 2014-11-13
• [Presentation] Raman Optical Activity of Peptides and Photoreceptor Proteins Analyzed by Quantum Chemical Calculations Combined with Molecular Dynamics Simulations (2014)
  • Author(s): Masashi Unno
  • Organizer: Fourth International Conference on Vibrational Optical Activity
  • Place of Presentation: Baoding, China
  • Year and Date: 2014-10-26 – 2014-10-29
  • Invited
• [Presentation] Active Site Structure of Photoactive Yellow protin with a Locked Chromophore Analoge Revealed by Near-Infrared Raman Optical Activity (2014)
  • Author(s): Takahito Shingae, Kensuke Kubota, Nicole D. Foster, Masato Kumauchi, Wouter D. Hoff, Masashi Unno
  • Organizer: Fourth International Conference on Vibrational Optical Activity
  • Place of Presentation: Baoding, China
  • Year and Date: 2014-10-26 – 2014-10-29
• [Presentation] Near-Infrared Raman Optical Activity of Photoactive Yellow Protein with a Locked Chromophore Analogue Analyzed by Quantum Chemical Calculations and Molecular Dynamics Simulations (2014)
  • Author(s): Shojiro Haraguchi, Takahito Shingae, Masato Kumauchi, Wouter D. Hoff, Masashi Unno
  • Organizer: Fourth International Conference on Vibrational Optical Activity
  • Place of Presentation: Baoding, China
  • Year and Date: 2014-10-26 – 2014-10-29
• [Presentation] 共鳴ラマン分光法によるシアノバクテリオクロームRcaE発色団のプロトン化状態の解明 (2014)
  • Author(s): ○小副川 晋介、広瀬 侑、池内昌彦、海野雅司
  • Organizer: 九重分光学関連夏季セミナー2014
  • Place of Presentation: 九重
  • Year and Date: 2014-07-25 – 2014-07-26
• [Book] 生命の辞典、共鳴ラマン光学法 (2016)
  • Author(s): 海野雅司
  • Publisher: 朝倉書店
• [Remarks] 佐賀大学 海野-藤澤研究室
  • URL: http://biophysics.chem.saga-u.ac.jp/
• [Remarks] 佐賀大学 海野-藤澤研究室
  • URL: http://biophysics.chem.saga-u.ac.jp/
• [Remarks] 佐賀大学海野研究室ホームページ
  • URL: http://biophysics.chem.saga-u.ac.jp/unno-lab/homu.html