| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Monolayer nanotubes, which are formed by self-assembly of synthetic lipids having an azobenzene as a photoresponsive unit, were able to stably storage enzymes in the nanochannels. The both end capping of the nanotubes with magnetic nanoparticles enabled us to not only prevent the release of the encapsulated enzymes but also magnetically recover the nanotubes from the reaction solutions. Such bioreactors composed of the organic/inorganic hybrid nanotubes and enzymes were controllable by an external light stimulus. The trans-to-cis isomerization of the azobenzene unit via UV irradiation led to start the enzymatic reaction based on the morphological transformation from the hybrid nanotubes to helical nanocoils with gaps for connecting of the nanochannels to the bulk solutions including substrates. The cis-to-trans reversal isomerization via VIS irradiation quenched the enzymatic reaction as the result of the morphological transformation from the helical nanocoils to the hybrid nanotubes.
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