| Project/Area Number |
26410142
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Akita University |
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Principal Investigator |
OGAWA NOBUAKI 秋田大学, その他部局等, 理事 (80169193)
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| Co-Investigator(Kenkyū-buntansha) |
藤原 一彦 秋田大学, 工学資源学研究科, 准教授 (10375222)
丹野 剛紀 秋田大学, 学内共同利用施設等, 准教授 (70390721)
松村 洋寿 秋田大学, 理工学研究科, 講師 (60741824)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 金属ナノ粒子 / サイズ排除クロマトグラフィー / 表面修飾 / ゲル濾過クロマトグラフィー |
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| Outline of Final Research Achievements |
Surface functionality of gold nanoparticles (AuNPs) with small organic compounds such as thiols and surfactants or biomolecules allows to control their surface properties. Thus, the surface functionalization and purification of the functionalized AuNPs form residual modification reagents are key processes in biological and therapeutic applications. Here, we devise a novel versatile methodology for surface functionalization of AuNPs through a purification of functionalized-AuNPs by using a gel filtration chromatography (GFC). We tested purification of a surface-functionalized AuNPs by bovine serum albumin (BSA)-and three different charged thiols (11-mercapto-1-undecanoic acid, 11-amino-1-undecane thiol and 1-undecane thiol), respectively. The surface-functionalized AuNPs were successfully separated with high recovery rates of more over than 90% for BSA and 85% for thiols.
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