Development of HPLC columns for ultra high performance separation of bio-based highly hydrophilic compounds
| Project/Area Number |
26410151
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Kyoto Institute of Technology |
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Principal Investigator |
Ikegami Tohru 京都工芸繊維大学, 分子化学系, 准教授 (20301252)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 親水性相互作用クロマトグラフィー / 液体クロマトグラフィー / キラル分離 / カラム充填剤 / 分離特性解析 / 新水性ポリマー / 重合修飾 / 分離性能 / 親水性ポリマー / シリカモノリス |
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| Outline of Final Research Achievements |
High performance / highly selective hydrophilic interaction (HILIC) type, ion exchange type, chiral separation type HPLC columns were prepared for monolithic and particle-packed type. They provided highly hydrophilic nature exceeding the performance limit of commercially available HILIC columns. (1) The relationship between the reaction conditions of the polymerization for surface modification of silica and the corresponding performance of the column were carefully examined to establish optimal modification conditions. (2) The correlation between the chain length of the introduced polymer chains and the separation performance and characteristics were examined. (3) HILIC type columns providing theoretical plate height 4-10 microns, 300,000 theoretical plates were prepared and applied to metabolome analysis. (4) A column having all of HILIC, cation exchange, anion exchange and chiral separation ability was prepared.
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Report
(4 results)
Research Products
(18 results)
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[Journal Article] Effect of pressure on the selectivity of polymeric C18 and C30 stationary phases in reversed-phase liquid chromatography. Increased separation of isomeric fatty acid methyl esters, triacylglycerols, and tocopherols at high pressure2014
Author(s)
Okusa, K., Iwasaki, Y., Kuroda, I., Miwa, S., Ohira, M., Nagai, T., Mizobe, H., Gotoh, N., Ikegami, T., McCalley, D.V., Tanaka, N.
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Journal Title
Journal of Chromatography A
Volume: 1339
Pages: 86-95
DOI
Related Report
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