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Establishment of Equilibrium Analysis Method Using Separation Analysis under the Concomitant of Side Reactions

Research Project

Project/Area Number 26410154
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Analytical chemistry
Research InstitutionThe University of Tokushima

Principal Investigator

Takayanagi Toshio  徳島大学, 大学院理工学研究部, 教授 (50263554)

Co-Investigator(Kenkyū-buntansha) 薮谷 智規  愛媛大学, 紙産業イノベーションセンター, 教授 (80335786)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywordsキャピラリー電気泳動 / 分離分析 / 酸解離定数 / トリフェニルメタン系陽イオン染料 / ジピクリルアミネートイオン / ヘキサメチレンテトラミン / 易分解性医薬品 / カルボキシエステラーゼ / キャピラリーゾーン電気泳動 / 速度論反応 / ヒドロクロロチアジド / 分解生成物 / 酵素反応 / ブプロピオン / プラバスタチン
Outline of Final Research Achievements

This study aimed at the establishment of the analysis method of fast equilibrium under the concomitant of side reactions or under the presence of the degraded species from the objective substance in an aqueous solution. Separation analysis by capillary zone electrophoresis (CZE) has been utilized to realize it. Gradually generated substances from the objective substance can be resolved by CZE, while the objective substance in fast equilibrium is detected as a single peak signal with its migration time / electrophoretic mobility of the weighted average of the equilibrium species.
Acid dissociation constants of easily degradable substances have successfully been determined by the proposed analysis method in the presence of the degraded species from the object substance. They were UV-degradable haloperidol, acid-degradable hexamethylenetetramine and pravastatin, as well as heat-degradable bupropion and hydrochlorothiazide.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (29 results)

All 2017 2016 2015 2014 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results,  Acknowledgement Compliant: 5 results) Presentation (19 results) (of which Int'l Joint Research: 2 results,  Invited: 3 results) Remarks (1 results)

  • [Journal Article] キャピラリーゾーン電気泳動法の電気泳動移動度を用いるヒドロクロロチアジドとその分解生成物の酸解離定数の決定2017

    • Author(s)
      高柳俊夫,磯田昌志,伊藤大地,水口仁志
    • Journal Title

      分析化学

      Volume: 66

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Determination of the Acid-Base Dissociation Constant of Acid-Degradable Hexamethylenetetramine by Capillary Zone Electrophoresis2016

    • Author(s)
      T. Takayanagi, N. Shimakami, M. Kurashina, H. Mizuguchi, T. Yabutani
    • Journal Title

      Analytical Sciences

      Volume: 32 Issue: 12 Pages: 1327-1332

    • DOI

      10.2116/analsci.32.1327

    • NAID

      130005433404

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Analysis of Acid Dissociation Equilibrium of Bupropion by Capillary Zone Electrophoresis After the Heat-Degradation2016

    • Author(s)
      T. Takayanagi, D. Itoh, H. Mizuguchi
    • Journal Title

      CHROMATOGRAPHY

      Volume: 37 Issue: 3 Pages: 105-109

    • DOI

      10.15583/jpchrom.2016.009

    • NAID

      130005277406

    • ISSN
      1342-8284, 1348-3315
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Analysis of Chemical Equilibrium of Silicon-Substituted Fluorescein and Its Application to Develop a Scaffold for Red Fluorescent Probes2015

    • Author(s)
      K. Hirabayashi, K. Hanaoka, T. Takayanagi, Y. Toki, T. Egawa, M. Kamiya, T. Komatsu, T. Ueno, T. Terai, K. Yoshida, M. Uchiyama, T. Nagano, and Y. Urano
    • Journal Title

      Analytical Chemistry

      Volume: 87(17) Issue: 17 Pages: 9061-9069

    • DOI

      10.1021/acs.analchem.5b02331

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Determination of Acid Dissociation Constant of Pravastatin under Degraded Conditions by Capillary Zone Electrophoresis2015

    • Author(s)
      T. Takayanagi, M. Amiya, N. Shimakami, and T. Yabutani
    • Journal Title

      Analytical Sciences

      Volume: 31 Issue: 11 Pages: 1193-1196

    • DOI

      10.2116/analsci.31.1193

    • NAID

      130005108594

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Equilibrium Analyses in Aqueous Solution under Accompanying Side Reactions by Capillary Zone Electrophoresis2015

    • Author(s)
      伊永隆史
    • Journal Title

      BUNSEKI KAGAKU

      Volume: 64 Issue: 2 Pages: 105-116

    • DOI

      10.2116/bunsekikagaku.64.105

    • NAID

      130004732542

    • ISSN
      0525-1931
    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Analysis of Acid Dissociation of Photo-degradable Haloperidol through the Measurement of Electrophoretic Mobility by Capillary Zone Electrophoresis2014

    • Author(s)
      島上夏美, 薮谷智規, 高柳俊夫
    • Journal Title

      BUNSEKI KAGAKU

      Volume: 63 Issue: 8 Pages: 643-648

    • DOI

      10.2116/bunsekikagaku.63.643

    • NAID

      130004687300

    • ISSN
      0525-1931
    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Analysis of Ion-association Equilibrium of Precipitable Dipicrylaminate Ion in Aqueous Solution by Capillary Zone Electrophoresis2014

    • Author(s)
      T. Takayanagi, K. Ogura, and T. Yabutani
    • Journal Title

      Analytical Sciences

      Volume: 30 Issue: 9 Pages: 919-924

    • DOI

      10.2116/analsci.30.919

    • NAID

      130004687419

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Analysis of Binding Equilibrium of Phenylalkylamines to Brij 58 Micelle through the Measurement of Electrophoretic Mobility by CZE2014

    • Author(s)
      T. Takayanagi, G. Inoue, A. Ikuta
    • Journal Title

      CHROMATOGRAPHY

      Volume: 35 Issue: 3 Pages: 147-154

    • DOI

      10.15583/jpchrom.2014.024

    • NAID

      130004715569

    • ISSN
      1342-8284, 1348-3315
    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] ポリエチレングリコールを添加したミセル動電クロマトグラフィーにおけるグラフェンの保持挙動とシグナル形状の変化2016

    • Author(s)
      高柳 俊夫,別役 優太,水口 仁志
    • Organizer
      第27回クロマトグラフィー科学会議
    • Place of Presentation
      慶応義塾大学(東京都港区)
    • Year and Date
      2016-11-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] キャピラリーゾーン電気泳動法を用いたブプロピオンの分解反応の追跡と分解条件下での酸解離平衡解析2016

    • Author(s)
      伊藤 大地,高柳 俊夫
    • Organizer
      第36回キャピラリー電気泳動シンポジウム
    • Place of Presentation
      徳島大学(徳島県徳島市)
    • Year and Date
      2016-11-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] キャピラリー電気泳動法を用いたエステラーゼによる酢酸p-ニトロフェニルの加水分解反応速度の解析2016

    • Author(s)
      岡田 英理子,高柳 俊夫
    • Organizer
      第36回キャピラリー電気泳動シンポジウム
    • Place of Presentation
      徳島大学(徳島県徳島市)
    • Year and Date
      2016-11-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] PEGを添加したミセル動電クロマトグラフィーにおけるグラフェン懸濁液の電気泳動2016

    • Author(s)
      別役 優太,水口 仁志,高柳 俊夫
    • Organizer
      第36回キャピラリー電気泳動シンポジウム
    • Place of Presentation
      徳島大学(徳島県徳島市)
    • Year and Date
      2016-11-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] グラフェン懸濁液のミセル動電クロマトグラフィーにおけるポリエチレングリコールの添加効果2016

    • Author(s)
      別役 優太,倉科 昌,高柳 俊夫
    • Organizer
      第76回 分析化学討論会
    • Place of Presentation
      岐阜大学(岐阜県岐阜市)
    • Year and Date
      2016-05-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] キャピラリー電気泳動法を用いたエステラーゼの加水分解反応の速度論解析2016

    • Author(s)
      岡田英理子, 高柳俊夫
    • Organizer
      第76回分析化学討論会
    • Place of Presentation
      岐阜薬科大学・岐阜大学(岐阜県岐阜市)
    • Year and Date
      2016-05-28
    • Related Report
      2015 Research-status Report
  • [Presentation] キャピラリー電気泳動法による分解反応下でのブプロピオンの酸解離反応解析2016

    • Author(s)
      伊藤大地,高柳俊夫
    • Organizer
      第76回分析化学討論会
    • Place of Presentation
      岐阜薬科大学・岐阜大学(岐阜県岐阜市)
    • Year and Date
      2016-05-28
    • Related Report
      2015 Research-status Report
  • [Presentation] Micellar electrokinetic chromatography of graphenes2015

    • Author(s)
      Toshio Takayanagi, Masaki Morimoto, Yuki Tomiyama, and Tomoki Yabutani
    • Organizer
      PACIFICHEM 2015
    • Place of Presentation
      Honolulu, Hawaii, USA
    • Year and Date
      2015-12-15
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Determination of acid dissociation constants of phenolphthalein and its derivatives under degradation2015

    • Author(s)
      Toshio Takayanagi
    • Organizer
      PACIFICHEM 2015
    • Place of Presentation
      Honolulu, Hawaii, USA
    • Year and Date
      2015-12-15
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] キャピラリーゾーン電気泳動分離を活用する易分解性物質の反応物性解析2015

    • Author(s)
      高柳俊夫
    • Organizer
      第26回クロマトグラフィー科学会議
    • Place of Presentation
      九州大学(福岡県福岡市)
    • Year and Date
      2015-11-11
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] キャピラリーゾーン電気泳動法によるヘキサメチレンテトラミンの酸解離反応解析2015

    • Author(s)
      島上夏美,薮谷智規,高柳俊夫
    • Organizer
      第35回キャピラリー電気泳動シンポジウム
    • Place of Presentation
      岡山大学(岡山県岡山市)
    • Year and Date
      2015-11-04
    • Related Report
      2015 Research-status Report
  • [Presentation] キャピラリーゾーン電気泳動法を用いたヘキサメチレンテトラミンの酸解離反応解析2015

    • Author(s)
      島上夏美,薮谷智規,高柳俊夫
    • Organizer
      第75回分析化学討論会
    • Place of Presentation
      山梨大学(山梨県甲府市)
    • Year and Date
      2015-05-23
    • Related Report
      2015 Research-status Report
  • [Presentation] Micellar Electrokinetic Chromatography of Graphenes with Polymer Additive2014

    • Author(s)
      T. Takayanagi, Y. Tomiyama, M. Morimoto, T. Yabutani
    • Organizer
      14th Asia-Pacific International Symposium on Microscale Separations and Analysis
    • Place of Presentation
      京都大学桂キャンパス(京都府京都市)
    • Year and Date
      2014-12-07 – 2014-12-10
    • Related Report
      2014 Research-status Report
  • [Presentation] Determination of Acid Dissociation Constant of Haloperidol under Photo-degraded Conditions through the Measurement of Electrophoretic Mobility by Capillary Zone Electrophoresis2014

    • Author(s)
      N. Shimakami, T. Yabutani, T. Takayanagi
    • Organizer
      14th Asia-Pacific International Symposium on Microscale Separations and Analysis
    • Place of Presentation
      京都大学桂キャンパス(京都府京都市)
    • Year and Date
      2014-12-07 – 2014-12-10
    • Related Report
      2014 Research-status Report
  • [Presentation] キャピラリーゾーン電気泳動法を用いた光分解性ハロペリドールの酸解離反応解析2014

    • Author(s)
      島上夏美,薮谷智規,高柳俊夫
    • Organizer
      日本分析化学会第63年会
    • Place of Presentation
      広島大学(広島県東広島市)
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Research-status Report
  • [Presentation] キャピラリー電気泳動法によるポリエーテル系非イオン界面活性剤ミセルへの結合反応解析とイオン種結合の選択性2014

    • Author(s)
      高柳俊夫
    • Organizer
      日本分析化学会第63年会
    • Place of Presentation
      広島大学(広島県東広島市)
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Equilibrium Analysis by Capillary Zone Electrophoresis - Its Characteristics and Advantages2014

    • Author(s)
      T. Takayanagi
    • Organizer
      1st Asian Symposium on Analytical Sciences
    • Place of Presentation
      広島大学(広島県東広島市)
    • Year and Date
      2014-09-17
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] Inhibitor Assay of Xanthine Oxidase by Photometric Flow Injection Analysis with Bindschedler's Green Leuco Base2014

    • Author(s)
      A. Kimura, T. Yabutani, T. Takayanagi
    • Organizer
      RSC Tokyo Innternational Conference,JASIS conference
    • Place of Presentation
      幕張メッセ(千葉県千葉市)
    • Year and Date
      2014-09-04 – 2014-09-05
    • Related Report
      2014 Research-status Report
  • [Presentation] グラフェンのミセル動電クロマトグラフィーにおける高分子添加の効果2014

    • Author(s)
      高柳俊夫,富山裕紀,薮谷智規
    • Organizer
      第21回クロマトグラフィーシンポジウム
    • Place of Presentation
      名古屋市工業研究所(愛知県名古屋市)
    • Year and Date
      2014-06-04 – 2014-06-06
    • Related Report
      2014 Research-status Report
  • [Remarks] 徳島大学大学院 先端技術科学教育部 物質生命システム工学専攻 分析・環境化学B-1講座ホームページ

    • URL

      http://www.chem.tokushima-u.ac.jp/B1/index.html

    • Related Report
      2016 Annual Research Report 2015 Research-status Report 2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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