Development of PCR-free electrochemical detection of micro RNAs
| Project/Area Number |
26410155
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Kyushu Institute of Technology |
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Principal Investigator |
Sato Shinobu 九州工業大学, 大学院工学研究院, 准教授 (80510677)
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| Research Collaborator |
HAYASHIDA Yasunobu
NAKAYAMA Akira
ONO Mizuki
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | miRNA / 電気化学遺伝子検出 / フェロセン化ナフタレンジイミド / DNA・RNA2本鎖 / 非増幅系検出 / 電気化学的遺伝子検出 / インターカレータ |
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| Outline of Final Research Achievements |
It is known that micro RNAs (miRNAs) are associated with an expression of tumor suppressor genes and are expecting to be one of good cancer markers. Here, we developed ferrocenylnaphthalene diimide (FND)-based hybridization assay to analyze specific miRNA without any amplification step. Since our previous research demonstrated that FND can bind to DNA-RNA hetero duplex, we constructed miRNA detecting system using FND coupled with an electrode chip carrying DNA probe for target miRNA. Here, we chose the suitable FND derivatives (FND1-7) to detect the miRNA by using Tm measurement and try to detect of target miRNA by using electrochemical hybridization assay. FND3 has the highest stabilization ability for DNA-RNA duplex and gave an increasing current shift with target miRNA in this hybridization assay, whereas almost no current shift was observed with non-target miRNA.
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Report
(4 results)
Research Products
(15 results)
