| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
A digital electrophoresis device was newly developed to improve resolution and sensitivity of protein analysis in complex biogenic samples as compared to conventional western blotting assay. The developed capillary cartridges filled with hydrogels having different functional groups were easily connected each other, providing the designable digital electrophoresis device containing various separation modes. The digital molecular sieving separation, digital isoelectric fractionation, and digital affinity separation were successfully conducted by the developed devices. The combination of these modes were also confirmed, which indicated that digital western blotting could be conducted by using the proposed digital electrophoresis device.
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